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- Keyword
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- Acetogenesis (6)
- Acetyl-CoA pathway (6)
- Moorella thermoacetica (6)
- Oxalate (6)
- Acidification (5)
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- PH (5)
- Acetogenic bacteria (4)
- Autotrophy (4)
- Bioenergetics (4)
- Electron transport (4)
- Nitrate dissimilation (4)
- Carbon source (3)
- Oxalate synthesis (3)
- Phytopathogenic fungus (3)
- Regulation (3)
- Sclerotinia sclerotiorum (3)
- CELL-mediated cytotoxicity (2)
- CELLS (2)
- CO2 fixation (2)
- CYTOLOGY (2)
- Clostridium aceticum (2)
- Clostridium thermoaceticum (2)
- DOSE-RESPONSE relationship (2)
- Diseases & pests (2)
- FUNGICIDES (2)
- Foliar bacterial pathogen (2)
- Forest soils; Anaerobes; Oxalate consumption; Organic acids; Acetate formation; Carbon cycling (2)
- Intercycle coupling (2)
- MAMMALS (2)
- Monosaccharides (2)
Articles 1 - 21 of 21
Full-Text Articles in Life Sciences
Evaluation Of Glyphosate-Tolerant Soybean Cultivars For Resistance To Bacterial Pustule, Lopa Goradia, Glen Hartman, Steven Daniel
Evaluation Of Glyphosate-Tolerant Soybean Cultivars For Resistance To Bacterial Pustule, Lopa Goradia, Glen Hartman, Steven Daniel
Faculty Research & Creative Activity
Xanthomonas axonopodis pv. glycines causes bacterial pustule of soybean, which is a common disease in many soybean-growing areas of the world and is controlled by a single recessive gene (rxp gene) commonly found in many conventional glyphosate-sensitive soybean cultivars. Since glyphosate-tolerant cultivars are commonly planted today, there has been no information about whether these new cultivars have bacterial pustule resistance. The goal of this study was to screen glyphosate-tolerant soybean cultivars for resistance to X. axonopodis pv. glycines. Three experiments were completed to evaluate resistance. In experiment 1, 525 commercial glyphosate-tolerant cultivars from 2001 were inoculated with X. axonopodis pv. …
Evaluation Of Glyphosate-Tolerant Soybean Cultivars For Resistance To Bacterial Pustule, Lopa Goradia, Glen Hartman, Steven L. Daniel
Evaluation Of Glyphosate-Tolerant Soybean Cultivars For Resistance To Bacterial Pustule, Lopa Goradia, Glen Hartman, Steven L. Daniel
Faculty Research & Creative Activity
Xanthomonas axonopodis pv. glycines causes bacterial pustule of soybean, which is a common disease in many soybean-growing areas of the world and is controlled by a single recessive gene (rxp gene) commonly found in many conventional glyphosate-sensitive soybean cultivars. Since glyphosate-tolerant cultivars are commonly planted today, there has been no information about whether these new cultivars have bacterial pustule resistance. The goal of this study was to screen glyphosate-tolerant soybean cultivars for resistance to X. axonopodis pv. glycines. Three experiments were completed to evaluate resistance. In experiment 1, 525 commercial glyphosate-tolerant cultivars from 2001 were inoculated with X. axonopodis pv. …
Old Acetogens, New Light, Steven L. Daniel, Harold L. Drake, Anita S. Gößner
Old Acetogens, New Light, Steven L. Daniel, Harold L. Drake, Anita S. Gößner
Faculty Research & Creative Activity
Acetogens utilize the acetyl-CoA Wood-Ljungdahl pathway as a terminal electron-accepting, energy-conserving, CO2-fixing process. The decades of research to resolve the enzymology of this pathway (1) preceded studies demonstrating that acetogens not only harbor a novel CO2-fixing pathway, but are also ecologically important, and (2) overshadowed the novel microbiological discoveries of acetogens and acetogenesis. The first acetogen to be isolated, Clostridium aceticum, was reported by Klaas Tammo Wieringa in 1936, but was subsequently lost. The second acetogen to be isolated, Clostridium thermoaceticum, was isolated by Francis Ephraim Fontaine and co-workers in 1942. C. thermoaceticum became the most extensively studied acetogen and …
Old Acetogens, New Light, Steven Daniel, Harold Drake, Anita Gößner
Old Acetogens, New Light, Steven Daniel, Harold Drake, Anita Gößner
Faculty Research & Creative Activity
Acetogens utilize the acetyl-CoA Wood-Ljungdahl pathway as a terminal electron-accepting, energy-conserving, CO2-fixing process. The decades of research to resolve the enzymology of this pathway (1) preceded studies demonstrating that acetogens not only harbor a novel CO2-fixing pathway, but are also ecologically important, and (2) overshadowed the novel microbiological discoveries of acetogens and acetogenesis. The first acetogen to be isolated, Clostridium aceticum, was reported by Klaas Tammo Wieringa in 1936, but was subsequently lost. The second acetogen to be isolated, Clostridium thermoaceticum, was isolated by Francis Ephraim Fontaine and co-workers in 1942. C. thermoaceticum became the most extensively studied acetogen and …
Impact Of Carbon Sources On Growth And Oxalate Synthesis By The Phytopathogenic Fungus Sclerotinia Sclerotiorum, Bryan J. Culbertson, Jaymie Krone, Erastus Gatebe, Norbert C. Furumo, Steven L. Daniel
Impact Of Carbon Sources On Growth And Oxalate Synthesis By The Phytopathogenic Fungus Sclerotinia Sclerotiorum, Bryan J. Culbertson, Jaymie Krone, Erastus Gatebe, Norbert C. Furumo, Steven L. Daniel
Faculty Research & Creative Activity
The impact of various supplemental carbon sources (oxalate, glyoxylate, glycolate, pyruvate, formate, malate, acetate, and succinate) on growth and oxalate formation (i.e., oxalogenesis) by Sclerotinia sclerotiorum was studied. With isolates D-E7, 105, W-B10, and Arg-L of S. sclerotiorum, growth in an undefined broth medium (0.1% soytone; pH 5) with 25 mM glucose and 25 mM supplemental carbon source was increased by the addition of malate and succinate. Oxalate accumulation occurred in the presence of glucose and a supplemental carbon source, with malate, acetate, and succinate supporting the most oxalate synthesis. With S. sclerotiorum Arg-L, oxalate-to-biomass ratios, an indicator of oxalogenic …
Impact Of Carbon Sources On Growth And Oxalate Synthesis By The Phytopathogenic Fungus Sclerotinia Sclerotiorum, Steven L. Daniel, Bryan J. Culbertson, Jaymie Krone, Norbert Furumo
Impact Of Carbon Sources On Growth And Oxalate Synthesis By The Phytopathogenic Fungus Sclerotinia Sclerotiorum, Steven L. Daniel, Bryan J. Culbertson, Jaymie Krone, Norbert Furumo
Faculty Research & Creative Activity
The impact of various supplemental carbon sources (oxalate, glyoxylate, glycolate, pyruvate, formate, malate, acetate, and succinate) on growth and oxalate formation (i.e., oxalogenesis) by Sclerotinia sclerotiorum was studied. With isolates D-E7, 105, W-B10, and Arg-L of S. sclerotiorum, growth in an undefined broth medium (0.1% soytone; pH 5) with 25 mM glucose and 25 mM supplemental carbon source was increased by the addition of malate and succinate. Oxalate accumulation occurred in the presence of glucose and a supplemental carbon source, with malate, acetate, and succinate supporting the most oxalate synthesis. With S. sclerotiorum Arg-L, oxalate-to-biomass ratios, an indicator of oxalogenic …
Impact Of Carbon Sources On Growth And Oxalate Synthesis By The Phytopathogenic Fungus Sclerotinia Sclerotiorum, Bryan Culbertson, Jaymie Krone, Erastus Gatebe, Norbert Furumo, Steven Daniel
Impact Of Carbon Sources On Growth And Oxalate Synthesis By The Phytopathogenic Fungus Sclerotinia Sclerotiorum, Bryan Culbertson, Jaymie Krone, Erastus Gatebe, Norbert Furumo, Steven Daniel
Faculty Research & Creative Activity
The impact of various supplemental carbon sources (oxalate, glyoxylate, glycolate, pyruvate, formate, malate, acetate, and succinate) on growth and oxalate formation (i.e., oxalogenesis) by Sclerotinia sclerotiorum was studied. With isolates D-E7, 105, W-B10, and Arg-L of S. sclerotiorum, growth in an undefined broth medium (0.1% soytone; pH 5) with 25 mM glucose and 25 mM supplemental carbon source was increased by the addition of malate and succinate. Oxalate accumulation occurred in the presence of glucose and a supplemental carbon source, with malate, acetate, and succinate supporting the most oxalate synthesis. With S. sclerotiorum Arg-L, oxalate-to-biomass ratios, an indicator of oxalogenic …
Mammalian Cell Cytotoxicity Analysis Of Soybean Rust Fungicides, Steven Daniel, G. Hartman, E. Wagner, M. Plewa
Mammalian Cell Cytotoxicity Analysis Of Soybean Rust Fungicides, Steven Daniel, G. Hartman, E. Wagner, M. Plewa
Faculty Research & Creative Activity
No abstract provided.
Mammalian Cell Cytotoxicity Analysis Of Soybean Rust Fungicides, Steven L. Daniel, G. L. Hartman, E. D. Wagner, M. J. Plewa
Mammalian Cell Cytotoxicity Analysis Of Soybean Rust Fungicides, Steven L. Daniel, G. L. Hartman, E. D. Wagner, M. J. Plewa
Faculty Research & Creative Activity
No abstract provided.
Impact Of Nutritional Supplements And Monosaccharides On Growth, Oxalate Accumulation, And Culture Ph By Sclerotinia Sclerotiorum, Steven Daniel, Bryan Culbertson, Norbert Furumo
Impact Of Nutritional Supplements And Monosaccharides On Growth, Oxalate Accumulation, And Culture Ph By Sclerotinia Sclerotiorum, Steven Daniel, Bryan Culbertson, Norbert Furumo
Faculty Research & Creative Activity
Sclerotinia sclerotiorum D-E7 was studied to determine the impact of nutritional supplements and monosaccharides on growth, oxalate accumulation, and culture pH in broth media (initial pH c. 5). Cultures with 0.1% nutritional supplement (tryptone, yeast extract, or soytone) yielded minimal growth, 2–3 mM oxalate, and a final culture pH of 4.2–4.8. In contrast, cultures with 0.1% nutritional supplement and 25 mM glucose yielded significant growth, minimal oxalate (<1 mM), and a final culture pH of 2.8–3.7. Similar trends were observed when glucose in 0.1% soytone cultures was replaced with 25 mM d-mannose, l-arabinose, or d-xylose. With 1% soytone-25 mM glucose cultures, growth and oxalate accumulation (∼21 mM) occurred with little change in initial pH. This was not the case with 1% soytone-250 mM glucose cultures; increased glucose levels resulted in a decrease in oxalate accumulation (∼7 mM) and in final culture pH (3.4). Time-course studies with these cultures revealed that oxalate accumulation was suppressed during growth when the culture pH dropped to <4. Overall, these results indicate that (1) the decrease in external pH (i.e. acidification) was independent of oxalate accumulation and (2) acidification coupled to glucose-dependent growth regulated oxalate accumulation by Sclerotinia sclerotiorum.
Impact Of Nutritional Supplements And Monosaccharides On Growth, Oxalate Accumulation, And Culture Ph By Sclerotinia Sclerotiorum, Steven L. Daniel, Bryan J. Culbertson, Norbert C. Furumo
Impact Of Nutritional Supplements And Monosaccharides On Growth, Oxalate Accumulation, And Culture Ph By Sclerotinia Sclerotiorum, Steven L. Daniel, Bryan J. Culbertson, Norbert C. Furumo
Faculty Research & Creative Activity
Sclerotinia sclerotiorum D-E7 was studied to determine the impact of nutritional supplements and monosaccharides on growth, oxalate accumulation, and culture pH in broth media (initial pH c. 5). Cultures with 0.1% nutritional supplement (tryptone, yeast extract, or soytone) yielded minimal growth, 2–3 mM oxalate, and a final culture pH of 4.2–4.8. In contrast, cultures with 0.1% nutritional supplement and 25 mM glucose yielded significant growth, minimal oxalate (<1 mM), and a final culture pH of 2.8–3.7. Similar trends were observed when glucose in 0.1% soytone cultures was replaced with 25 mM d-mannose, l-arabinose, or d-xylose. With 1% soytone-25 mM glucose cultures, growth and oxalate accumulation (∼21 mM) occurred with little change in initial pH. This was not the case with 1% soytone-250 mM glucose cultures; increased glucose levels resulted in a decrease in oxalate accumulation (∼7 mM) and in final culture pH (3.4). Time-course studies with these cultures revealed that oxalate accumulation was suppressed during growth when the culture pH dropped to <4. Overall, these results indicate that (1) the decrease in external pH (i.e. acidification) was independent of oxalate accumulation and (2) acidification coupled to glucose-dependent growth regulated oxalate accumulation by Sclerotinia sclerotiorum.
Anaerobic Oxalate Consumption By Microorganisms In Forest Soils, Steven Daniel, Christine Pilsl, Harold Drake
Anaerobic Oxalate Consumption By Microorganisms In Forest Soils, Steven Daniel, Christine Pilsl, Harold Drake
Faculty Research & Creative Activity
The microbial consumption of oxalate was examined under anaerobic conditions in soil suspensions at 15-20 degree C. With soil (horizon Ah, pH 6.4) from a beech forest, microbial consumption of added oxalate (15 mM) began after 10 days, and oxalate was totally consumed by day 20. The presence of supplemental electron donors (acetate, glucose, vanillate, or hydrogen) or electron acceptors (nitrate or sulfate) did not significantly influence anaerobic oxalate consumption, whereas supplementation of soil suspensions with CO2/bicarbonate totally repressed oxalate consumption. Thus, CO2-, nitrate- or sulfate-respiring bacteria were apparently not active in the anaerobic consumption of oxalate in these soil …
Anaerobic Oxalate Consumption By Microorganisms In Forest Soils, Steven L. Daniel, Christine Pilsl, Harold L. Drake
Anaerobic Oxalate Consumption By Microorganisms In Forest Soils, Steven L. Daniel, Christine Pilsl, Harold L. Drake
Faculty Research & Creative Activity
The microbial consumption of oxalate was examined under anaerobic conditions in soil suspensions at 15-20 degree C. With soil (horizon Ah, pH 6.4) from a beech forest, microbial consumption of added oxalate (15 mM) began after 10 days, and oxalate was totally consumed by day 20. The presence of supplemental electron donors (acetate, glucose, vanillate, or hydrogen) or electron acceptors (nitrate or sulfate) did not significantly influence anaerobic oxalate consumption, whereas supplementation of soil suspensions with CO2/bicarbonate totally repressed oxalate consumption. Thus, CO2-, nitrate- or sulfate-respiring bacteria were apparently not active in the anaerobic consumption of oxalate in these soil …
Physiology Of The Thermophilic Acetogen Moorella Thermoacetica, Harold Drake, Steven Daniel
Physiology Of The Thermophilic Acetogen Moorella Thermoacetica, Harold Drake, Steven Daniel
Faculty Research & Creative Activity
Moorella thermoacetica (originally isolated as Clostridium thermoaceticum) has served as the primary acetogenic bacterium for the resolution of the acetyl coenzyme A (acetyl-CoA) orWood–Ljungdahl pathway, a metabolic pathway that (i) autotrophically assimilates CO2 and (ii) is centrally important to the turnover of carbon in many habitats. The purpose of this article is to highlight the diverse physiological features of this model acetogen and to examine some of the consequences of its metabolic capabilities.
Physiology Of The Thermophilic Acetogen Moorella Thermoacetica, Harold L. Drake, Steven L. Daniel
Physiology Of The Thermophilic Acetogen Moorella Thermoacetica, Harold L. Drake, Steven L. Daniel
Faculty Research & Creative Activity
Moorella thermoacetica (originally isolated as Clostridium thermoaceticum) has served as the primary acetogenic bacterium for the resolution of the acetyl coenzyme A (acetyl-CoA) orWood–Ljungdahl pathway, a metabolic pathway that (i) autotrophically assimilates CO2 and (ii) is centrally important to the turnover of carbon in many habitats. The purpose of this article is to highlight the diverse physiological features of this model acetogen and to examine some of the consequences of its metabolic capabilities.
Oxalate Metabolism By The Acetogenic Bacterium Moorella Thermoacetica, Steven Daniel, Christine Pilsl, Harold Drake
Oxalate Metabolism By The Acetogenic Bacterium Moorella Thermoacetica, Steven Daniel, Christine Pilsl, Harold Drake
Faculty Research & Creative Activity
Whole-cell and cell-extract experiments were performed to study the mechanism of oxalate metabolism in the acetogenic bacterium Moorella thermoacetica. In short-term, whole-cell assays, oxalate consumption was low unless cell suspensions were supplemented with CO2, KNO3, or Na2S2O3. Cell extracts catalyzed the oxalate-dependent reduction of benzyl viologen. Oxalate consumption occurred concomitant to benzyl viologen reduction; when benzyl viologen was omitted, oxalate was not appreciably consumed. Based on benzyl viologen reduction, specific activities of extracts averaged 0.6 μmol oxalate oxidized min−1 mg protein−1. Extracts also catalyzed the formate-dependent reduction of NADP+; however, oxalate-dependent reduction of NADP+ was negligible. Oxalate- or formate-dependent reduction …
Oxalate Metabolism By The Acetogenic Bacterium Moorella Thermoacetica, Steven L. Daniel, Christine Pilsl, Harold L. Drake
Oxalate Metabolism By The Acetogenic Bacterium Moorella Thermoacetica, Steven L. Daniel, Christine Pilsl, Harold L. Drake
Faculty Research & Creative Activity
Whole-cell and cell-extract experiments were performed to study the mechanism of oxalate metabolism in the acetogenic bacterium Moorella thermoacetica. In short-term, whole-cell assays, oxalate consumption was low unless cell suspensions were supplemented with CO2, KNO3, or Na2S2O3. Cell extracts catalyzed the oxalate-dependent reduction of benzyl viologen. Oxalate consumption occurred concomitant to benzyl viologen reduction; when benzyl viologen was omitted, oxalate was not appreciably consumed. Based on benzyl viologen reduction, specific activities of extracts averaged 0.6 μmol oxalate oxidized min−1 mg protein−1. Extracts also catalyzed the formate-dependent reduction of NADP+; however, oxalate-dependent reduction of NADP+ was negligible. Oxalate- or formate-dependent reduction …
Carbon Monoxide-Dependent Chemolithotrophic Growth Of Clostridium Thermoautotrophicum, M. Dean Savage, Zhongren Wu, Steven L. Daniel, Leon L. Lundie Jr, Harold L. Drake
Carbon Monoxide-Dependent Chemolithotrophic Growth Of Clostridium Thermoautotrophicum, M. Dean Savage, Zhongren Wu, Steven L. Daniel, Leon L. Lundie Jr, Harold L. Drake
Faculty Research & Creative Activity
The acetogen Clostridium thermoautotrophicum was cultivated under CO-dependent chemolithotrophic conditions. CO-dependent growth profiles and energetics indicated that supplemental CO2 was fundamental to efficient growth at the expense of CO. Overall product stoichiometry approximated 6.5CO --> CH3CO2H + 3.5CO2 + 0.6 cell C + 0.5 unrecovered C. Initial CO/CO2 ratios of 2 to 4 yielded optimal doubling times and cell yields. Maximal YCO values approximated 2.5 g of cell dry weight per mol of CO consumed; Y H2 , was considerably lower than Y CO Cross-transfer growth experiments and protein profiles indicated differential expression of genes between CO and methanol cultures.
Microbial Degradation Of Oxalate In The Gastrointestinal Tracts Of Rats, Steven L. Daniel
Microbial Degradation Of Oxalate In The Gastrointestinal Tracts Of Rats, Steven L. Daniel
Faculty Research & Creative Activity
Rates of oxalate degradation by mixed bacterial populations in cecal contents from wlld rats ranged from 2.5 to 20.6 µmol/g (dry weight) per h. The oxalate-degrading activity in cecal contents from three strains of laboratory rats (Long-Evans, Wistar, and Sprague-Dawley) from four commercial breeders was generally lower, ranging from 1.8 to 3.5 µmollg (dry weight) of cecal contents per h. This activity did not increase when diets were supplemented with oxalate. Wben Sprague-Pawley rats from a fifth commercial breeder were fed an oxalate diet, rates of oxalate degradation in cecal contents increased from 2.0 to 23.1 µmollg (dry weight) per …
Carbon Monoxide-Dependent Chemolithotrophic Growth Of Clostridium Thermoautotrophicum, M. Savage, Zhongren Wu, Steven Daniel, Leon Lundie, Harold Drake
Carbon Monoxide-Dependent Chemolithotrophic Growth Of Clostridium Thermoautotrophicum, M. Savage, Zhongren Wu, Steven Daniel, Leon Lundie, Harold Drake
Faculty Research & Creative Activity
The acetogen Clostridium thermoautotrophicum was cultivated under CO-dependent chemolithotrophic conditions. CO-dependent growth profiles and energetics indicated that supplemental CO2 was fundamental to efficient growth at the expense of CO. Overall product stoichiometry approximated 6.5CO --> CH3CO2H + 3.5CO2 + 0.6 cell C + 0.5 unrecovered C. Initial CO/CO2 ratios of 2 to 4 yielded optimal doubling times and cell yields. Maximal YCO values approximated 2.5 g of cell dry weight per mol of CO consumed; Y H2 , was considerably lower than Y CO Cross-transfer growth experiments and protein profiles indicated differential expression of genes between CO and methanol cultures.
Microbial Degradation Of Oxalate In The Gastrointestinal Tracts Of Rats, Steven Daniel
Microbial Degradation Of Oxalate In The Gastrointestinal Tracts Of Rats, Steven Daniel
Faculty Research & Creative Activity
Rates of oxalate degradation by mixed bacterial populations in cecal contents from wlld rats ranged from 2.5 to 20.6 µmol/g (dry weight) per h. The oxalate-degrading activity in cecal contents from three strains of laboratory rats (Long-Evans, Wistar, and Sprague-Dawley) from four commercial breeders was generally lower, ranging from 1.8 to 3.5 µmollg (dry weight) of cecal contents per h. This activity did not increase when diets were supplemented with oxalate. Wben Sprague-Pawley rats from a fifth commercial breeder were fed an oxalate diet, rates of oxalate degradation in cecal contents increased from 2.0 to 23.1 µmollg (dry weight) per …