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Full-Text Articles in Life Sciences
Evidence Of Western Corn Rootworm (Diabrotica Virgifera Virgifera Leconte) Field-Evolved Resistance To Cry3bb1 + Cry34/ 35ab1 Maize In Nebraska, Jordan D. Reinders, Emily E. Reinders, Emily Robinson, Bryan W. French, Lance J. Meinke
Evidence Of Western Corn Rootworm (Diabrotica Virgifera Virgifera Leconte) Field-Evolved Resistance To Cry3bb1 + Cry34/ 35ab1 Maize In Nebraska, Jordan D. Reinders, Emily E. Reinders, Emily Robinson, Bryan W. French, Lance J. Meinke
Department of Entomology: Faculty Publications
BACKGROUND: Western corn rootworm (WCR; Diabrotica virgifera virgifera) field-evolved resistance to transgenic maize expressing the Cry3Bb1 protein derived from Bacillus thuringiensis (Bt) has been confirmed across the United States Corn Belt. Although use of pyramided hybrids expressing Cry3Bb1 + Cry34/35Ab1 has increased in recent years to mitigate existing WCR Bt resistance, susceptibility of Nebraska WCR populations to this rootworm–Bt pyramid has not been assessed. Plant-based bioassays were used to characterize the susceptibility of WCR populations to Cry3Bb1 and Cry3Bb1 + Cry34/35Ab1 maize. Populations were collected from areas of northeastern Nebraska with a history of planting Bt maize that expressed …
The Regulation Landscape Of Mapk Signaling Cascade For Thwarting Bacillus Thuringiensis Infection In An Insect Host, Zhaojiang Guo, Shi Kang, Qingjun Wu, Shaoli Wang, Neil Crickmore, Xuguo Zhou, Alejandra Bravo, Mario Soberón, Youjun Zhang
The Regulation Landscape Of Mapk Signaling Cascade For Thwarting Bacillus Thuringiensis Infection In An Insect Host, Zhaojiang Guo, Shi Kang, Qingjun Wu, Shaoli Wang, Neil Crickmore, Xuguo Zhou, Alejandra Bravo, Mario Soberón, Youjun Zhang
Entomology Faculty Publications
Host-pathogen interactions are central components of ecological networks where the MAPK signaling pathways act as central hubs of these complex interactions. We have previously shown that an insect hormone modulated MAPK signaling cascade participates as a general switch to trans-regulate differential expression of diverse midgut genes in the diamondback moth, Plutella xylostella (L.) to cope with the insecticidal action of Cry1Ac toxin, produced by the entomopathogenic bacterium Bacillus thuringiensis (Bt). The relationship between topology and functions of this four-tiered phosphorylation signaling cascade, however, is an uncharted territory. Here, we carried out a genome-wide characterization of all the MAPK orthologs …
A Cis-Acting Mutation In The Pxabcg1 Promoter Is Associated With Cry1ac Resistance In Plutella Xylostella (L.), Jianying Qin, Fan Ye, Linzheng Xu, Xuguo Zhou, Neil Crickmore, Xiaomao Zhou, Youjun Zhang, Zhaojiang Guo
A Cis-Acting Mutation In The Pxabcg1 Promoter Is Associated With Cry1ac Resistance In Plutella Xylostella (L.), Jianying Qin, Fan Ye, Linzheng Xu, Xuguo Zhou, Neil Crickmore, Xiaomao Zhou, Youjun Zhang, Zhaojiang Guo
Entomology Faculty Publications
The molecular mechanisms of insect resistance to Cry toxins generated from the bacterium Bacillus thuringiensis (Bt) urgently need to be elucidated to enable the improvement and sustainability of Bt-based products. Although downregulation of the expression of midgut receptor genes is a pivotal mechanism of insect resistance to Bt Cry toxins, the underlying transcriptional regulation of these genes remains elusive. Herein, we unraveled the regulatory mechanism of the downregulation of the ABC transporter gene PxABCG1 (also called Pxwhite), a functional midgut receptor of the Bt Cry1Ac toxin in Plutella xylostella. The PxABCG1 promoters of Cry1Ac-susceptible and Cry1Ac-resistant strains were …