Life Sciences Commons^™

Historical Biogeography Of Velvet Ants (Hymenoptera: Mutillidae) In The North American Deserts And Arid Lands, Joseph S. Wilson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

For centuries, scientists have been intrigued by the high amount of biodiversity that is found in the deserts of North America. Recently, several studies have investigated the causes of the high diversity found in desert-dwelling mammals, reptiles, and amphibians. These studies have found that many of these organisms seem to have diversified in response to the same historical events. Little work has been done, however, on diverse desert-dwelling insect groups. In this dissertation, I investigate the patterns of genetic diversity in four groups of nocturnal wasps called velvet ants. I compare the patterns of genetic diversity to the historical events …

New Views On Strand Asymmetry In Insect Mitochondrial Genomes, Shu-Jun Wei, Min Shi, Xue-Xin Chen, Michael J. Sharkey, Cornelis Van Achterberg, Gong-Yin Ye, Jun-Hua He

Entomology Faculty Publications

Strand asymmetry in nucleotide composition is a remarkable feature of animal mitochondrial genomes. Understanding the mutation processes that shape strand asymmetry is essential for comprehensive knowledge of genome evolution, demographical population history and accurate phylogenetic inference. Previous studies found that the relative contributions of different substitution types to strand asymmetry are associated with replication alone or both replication and transcription. However, the relative contributions of replication and transcription to strand asymmetry remain unclear. Here we conducted a broad survey of strand asymmetry across 120 insect mitochondrial genomes, with special reference to the correlation between the signs of skew values and …

Comparative Mitogenomics Of Braconidae (Insecta: Hymenoptera) And The Phylogenetic Utility Of Mitochondrial Genomes With Special Reference To Holometabolous Insects, Shu-Jun Wei, Min Shi, Michael J. Sharkey, Cornelis Van Achterberg, Xue-Xin Chen

Entomology Faculty Publications

BACKGROUND: Animal mitochondrial genomes are potential models for molecular evolution and markers for phylogenetic and population studies. Previous research has shown interesting features in hymenopteran mitochondrial genomes. Here, we conducted a comparative study of mitochondrial genomes of the family Braconidae, one of the largest families of Hymenoptera, and assessed the utility of mitochondrial genomic data for phylogenetic inference at three different hierarchical levels, i.e., Braconidae, Hymenoptera, and Holometabola.

RESULTS: Seven mitochondrial genomes from seven subfamilies of Braconidae were sequenced. Three of the four sequenced A+T-rich regions are shown to be inverted. Furthermore, all species showed reversal of strand asymmetry, suggesting …

Purification And Characterization Of Cytoplasmic Nadp+- Isocitrate Dehydrogenase, And Amplification Of The Nadp+-Idh Gene From The Wing-Dimorphic Sand Field Cricket, Gryllus Firmus, Anthony J. Zera, Susan Newman, David Berkheim, Christine Black, Lindsay Klug, Erica Crone

School of Biological Sciences: Faculty Publications

Cytoplasmic NADP+-isocitrate dehydrogenase (NADP+-IDH) has been purified and characterized, and its gene sequenced in many animal, plant, and yeast species. However, much less information is available on this enzyme-gene in insects. As a first step in investigating the biochemical and molecular mechanisms by which NADP+-IDH contributes to adaptations for flight vs. reproduction in insects, the enzyme was purified to homogeneity in the wing-dimorphic cricket, Gryllus firmus, characterized, and its corresponding gene sequenced. Using a combination of polyethylene glycol precipitation, Cibacron-Blue affinity chromatography, and hydrophobic interaction chromatography the enzyme was purified 291-fold (7% yield; specific activity = 15.8 μmol NADPH/min/mg …