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Amplified Fragment Length Polymorphism Used For Inter- And Intraspecific Differentiation Of Screwworms (Diptera: Calliphoridae), L. Alamalakala, Steven R. Skoda, John E. Foster
Amplified Fragment Length Polymorphism Used For Inter- And Intraspecific Differentiation Of Screwworms (Diptera: Calliphoridae), L. Alamalakala, Steven R. Skoda, John E. Foster
Department of Entomology: Faculty Publications
Morphologically, early immature stages of the economically important pest called screwworms, Cochliomyia hominivorax (Coquerel) (Diptera: Calliphoridae), and non-pest secondary screwworms, Cochliomyia macellaria (Fabricius) (Diptera: Calliphoridae), are nearly indistinguishable. Correct identification is crucial to the ongoing eradication and exclusion program protecting the United States, Mexico and Central America from reinvasion of screwworms persistent in South America and the Caribbean. Amplified fragment length polymorphism (AFLP) polymerase chain reaction was used to differentiate populations of C. hominivorax and to discriminate them from C. macellaria. Ten primer pairs screened for interspecific discrimination of C. hominivorax from C. macellaria showed 52 discrete bands, allowing …