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Full-Text Articles in Life Sciences
Emerald Ash Borer Specific Gene Silencing Has No Effect On Non-Target Organisms, Flavia Pampolini, Lynne K. Rieske
Emerald Ash Borer Specific Gene Silencing Has No Effect On Non-Target Organisms, Flavia Pampolini, Lynne K. Rieske
Entomology Faculty Publications
The sequence complementarity of the RNA interference (RNAi) pathway allows for targeted suppression of genes essential for insect survival, and enables development of pest management strategies specific to a given species while reducing the likelihood of adversely impacting non-target organisms (NTOs). The feasibility of manipulating the RNAi pathway to cause mortality in the highly invasive emerald ash borer (EAB) has been demonstrated. Here the spectrum of activity of three double stranded RNAs (dsRNAs) targeting the genes hsp, shi, and sn-rnp in EAB was evaluated in model insects representing five functional guilds including herbivore, predator, detritivore, pollinator, parasitoid; …
Oral Ingestion Of Bacterially Expressed Dsrna Can Silence Genes And Cause Mortality In A Highly Invasive, Tree-Killing Pest, The Emerald Ash Borer, Ramya Shanivarsanthe Leelesh, Lynne K. Rieske
Oral Ingestion Of Bacterially Expressed Dsrna Can Silence Genes And Cause Mortality In A Highly Invasive, Tree-Killing Pest, The Emerald Ash Borer, Ramya Shanivarsanthe Leelesh, Lynne K. Rieske
Entomology Faculty Publications
RNA interference (RNAi) is a naturally occurring process inhibiting gene expression, and recent advances in our understanding of the mechanism have allowed its development as a tool against insect pests. A major challenge for deployment in the field is the development of convenient and efficient methods for production of double stranded RNA (dsRNA). We assessed the potential for deploying bacterially produced dsRNA as a bio-pesticide against an invasive forest pest, the emerald ash borer (EAB). EAB feeds on the cambial tissue of ash trees (Fraxinus spp.), causing rapid death. EAB has killed millions of trees in North America since …