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Full-Text Articles in Life Sciences
Molecular Gut Content Analysis Of Different Spider Body Parts, Nuria Macías-Hernández, Kacie J. Athey, Vanina Tonzo, Owen S. Wangensteen, Miquel Arnedo, James D Harwood
Molecular Gut Content Analysis Of Different Spider Body Parts, Nuria Macías-Hernández, Kacie J. Athey, Vanina Tonzo, Owen S. Wangensteen, Miquel Arnedo, James D Harwood
Entomology Faculty Publications
Molecular gut-content analysis has revolutionized the study of food webs and feeding interactions, allowing the detection of prey DNA within the gut of many organisms. However, successful prey detection is a challenging procedure in which many factors affect every step, starting from the DNA extraction process. Spiders are liquid feeders with branched gut diverticula extending into their legs and throughout the prosoma, thus digestion takes places in different parts of the body and simple gut dissection is not possible. In this study, we investigated differences in prey detectability in DNA extracts from different parts of the spider´s body: legs, prosoma …
Advancing Behavioural Genomics By Considering Timescale, Clare C. Rittschof, Kimberly A. Hughes
Advancing Behavioural Genomics By Considering Timescale, Clare C. Rittschof, Kimberly A. Hughes
Entomology Faculty Publications
Animal behavioural traits often covary with gene expression, pointing towards a genomic constraint on organismal responses to environmental cues. This pattern highlights a gap in our understanding of the time course of environmentally responsive gene expression, and moreover, how these dynamics are regulated. Advances in behavioural genomics explore how gene expression dynamics are correlated with behavioural traits that range from stable to highly labile. We consider the idea that certain genomic regulatory mechanisms may predict the timescale of an environmental effect on behaviour. This temporally minded approach could inform both organismal and evolutionary questions ranging from the remediation of early …
Creb-Binding Protein Plays Key Roles In Juvenile Hormone Action In The Red Flour Beetle, Tribolium Castaneum, Jingjing Xu, Amit Roy, Subba Reddy Palli
Creb-Binding Protein Plays Key Roles In Juvenile Hormone Action In The Red Flour Beetle, Tribolium Castaneum, Jingjing Xu, Amit Roy, Subba Reddy Palli
Entomology Faculty Publications
Juvenile hormones (JH) and ecdysteroids regulate many biological and metabolic processes. CREB-binding protein (CBP) is a transcriptional co-regulator with histone acetyltransferase (HAT) activity. Therefore, CBP is involved in activation of many transcription factors that regulate expression of genes associated with postembryonic development in insects. However, the function of CBP in JH action in insects is not well understood. Hence, we studied the role of CBP in JH action in the red flour beetle, Tribolium castaneum and the Tribolium cell line. CBP knockdown caused a decrease in JH induction of genes, Kr-h1, 4EBP and G13402 in T. castaneum larvae, adults and …
Selection Of Housekeeping Genes And Demonstration Of Rnai In Cotton Leafhopper, Amrasca Biguttula Biguttula (Ishida), Satnam Singh, Mridula Gupta, Suneet Pandher, Gurmeet Kaur, Pankaj Rathore, Subba Reddy Palli
Selection Of Housekeeping Genes And Demonstration Of Rnai In Cotton Leafhopper, Amrasca Biguttula Biguttula (Ishida), Satnam Singh, Mridula Gupta, Suneet Pandher, Gurmeet Kaur, Pankaj Rathore, Subba Reddy Palli
Entomology Faculty Publications
Amrasca biguttula biguttula (Ishida) commonly known as cotton leafhopper is a severe pest of cotton and okra. Not much is known on this insect at molecular level due to lack of genomic and transcriptomic data. To prepare for functional genomic studies in this insect, we evaluated 15 common housekeeping genes (Tub, B-Tub, EF alpha, GADPH, UbiCF, RP13, Ubiq, G3PD, VATPase, Actin, 18s, 28s, TATA, ETF, SOD and Cytolytic actin) during different developmental stages and under starvation stress. We selected early (1st and 2 …