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Reference Gene Selection For Transcriptional Profiling In Cryptocercus Punctulatus, An Evolutionary Link Between Isoptera And Blattodea, Zhen Li, Xiangrui Li, Qingwen Zhang, Ling Yuan, Xuguo Zhou

Plant and Soil Sciences Faculty Publications

The subsocial life style and wood-feeding capability of Cryptocercus gives us an evolutionary key to unlock some outstanding questions in biology. With the advent of the Genomics Era, there is an unprecedented opportunity to address the evolution of eusociality and the acquisition of lignocellulases at the genetic level. However, to quantify gene expression, an appropriate normalization strategy is warranted to control for the non-specific variations among samples across different experimental conditions. To search for the internal references, 10 housekeeping genes from a gut transcriptome of a wood-feeding cockroach, Cryptocercus punctulatus, were selected as the candidates for the RT-qPCR analysis. The …