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Lyophilization Of Spodoptera Frugiperda (Lepidoptera: Noctuidae) Larvae Yields High-Quality Dna For Use In Aflp Genetic Studies, Pete L. Clark, David J. Isenhour, Steven R. Skoda, Jaime Molina-Ochoa, Claudia Gianni, John E. Foster
Lyophilization Of Spodoptera Frugiperda (Lepidoptera: Noctuidae) Larvae Yields High-Quality Dna For Use In Aflp Genetic Studies, Pete L. Clark, David J. Isenhour, Steven R. Skoda, Jaime Molina-Ochoa, Claudia Gianni, John E. Foster
John E. Foster
Agricultural research in the 21st century has become a collaborative effort. Research on crop pests like Spodoptera frugiperda (J.E. Smith), commonly known as the fall armyworm (FAW), can involve international collaboration because it is a pest not only in the southern United States, but also in Latin and South America. Our interest to study the genetic variation of 24 subpopulations of FAW from the southern United States, Mexico, Puerto Rico, Brazil and Argentina required insect collection procedures that preserve the integrity of DNA for molecular genetic analysis. The samples were collected primarily from maize (Zea mays L.), but also included …
Genetic Structure And Molecular Variability Of Spodoptera Frugiperda (Lepidoptera: Noctuidae) Collected In Maize And Cotton Fields In Brazil, S. Martinelli, P. L. Clark, M. I. Zucchi, M. C. Silva-Filho, John E. Foster, C. Omoto
Genetic Structure And Molecular Variability Of Spodoptera Frugiperda (Lepidoptera: Noctuidae) Collected In Maize And Cotton Fields In Brazil, S. Martinelli, P. L. Clark, M. I. Zucchi, M. C. Silva-Filho, John E. Foster, C. Omoto
John E. Foster
The purpose of this research was to evaluate the genetic similarity and structure of the fall armyworm, Spodoptera frugiperda (J.E. Smith), populations associated with maize and cotton crops in Brazil using amplified fragment length polymorphisms. Mean genetic similarity among populations was 0.45. The unweighted pair group method with arithmetic mean analysis dendrograms did not separate populations of S. frugiperda into clusters related to the host plant in which the insects were collected. No genetic variation was observed among maize and cotton populations of S. frugiperda, suggesting that the same populations are injuring both crops in Brazil. This research validates the …