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- Borrelia burgdorferi (2)
- Dermacentor variabilis (2)
- Amplicon (1)
- Antimicrobial assays (1)
- Bacillus subtilis (1)
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- Blood Proteins (1)
- DNA Probes (1)
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- Ixodidae (1)
- Juvenile hormones (1)
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- Micrococcus luteus (1)
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- Peptide mapping (1)
- Preprodefensin (1)
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- Rabbits (1)
- Secretion (1)
- Tick (1)
- Vitellin (1)
- Vitellogenin (1)
Articles 1 - 3 of 3
Full-Text Articles in Life Sciences
Host Blood Proteins And Peptides In The Midgut Of The Tick Dermacentor Variabilis Contribute To Bacterial Control, Daniel E. Sonenshine, Wayne L. Hynes, Shane M. Ceraul, Robert Mitchell, Tiffany Benzine
Host Blood Proteins And Peptides In The Midgut Of The Tick Dermacentor Variabilis Contribute To Bacterial Control, Daniel E. Sonenshine, Wayne L. Hynes, Shane M. Ceraul, Robert Mitchell, Tiffany Benzine
Biological Sciences Faculty Publications
Antimicrobial midgut proteins and peptides that result from blood digestion in feeding American dog ticks Dermacentor variabilis (Say) were identified. Midgut extracts from these ticks showed antimicrobial activity against Micrococcus luteus, regardless of whether they were challenged with peptidoglycan, blood meal components, rabbit blood, Bacillus subtilis, Escherischia coli or Borrelia burgdorferi. However, no peptide band co-migrating with defensin was found in midgut extracts from the challenged ticks. Partial purification of the midgut extracts using C18 Sep Paks and gel electrophoresis showed the presence of 4 distinct bands with rMW 4.1, 5.3, 5.7 and 8.0 kDa identified …
In Vivo Role Of 20-Hydroxyecdysone In The Regulation Of The Vitellogenin Mrna And Egg Development In The American Dog Tick, Dermacentor Variabilis (Say), Deborah M. Thompson, Sayed M.S. Khalil, Laura A. Jeffers, Usha Ananthapadadmanaban, Daniel E. Sonenshine, Robert D. Mitchell, Christopher J. Osgood, Charles S. Apperson, R. Michael Roe
In Vivo Role Of 20-Hydroxyecdysone In The Regulation Of The Vitellogenin Mrna And Egg Development In The American Dog Tick, Dermacentor Variabilis (Say), Deborah M. Thompson, Sayed M.S. Khalil, Laura A. Jeffers, Usha Ananthapadadmanaban, Daniel E. Sonenshine, Robert D. Mitchell, Christopher J. Osgood, Charles S. Apperson, R. Michael Roe
Biological Sciences Faculty Publications
Injection of the hormone 20-hydroxyecdysone (20-E) into partially fed (virgin) female adults of the American dog tick, Dermacentor variabilis, while they are attached and feeding on the rabbit host, initiated the expression of the vitellogenin (Vg) gene, and Vg protein secretion and uptake by the ovary. The induction of egg production by 20-E in this bioassay was dose dependent in the range of 1-50 times the concentration normally found in a replete, vitellogenic female. Ticks examined 4d after the 50x treatment were still attached to the host, had numerous enlarged vitellin-filled (brown) oocytes in their ovaries, but had not …
Defensin In Ticks: Molecular Characterization, Expression, And Role In Innate Immunity, Daniel E. Sonenshine, Wayne L. Hynes, Shane M. Ceraul, Michelle Todd
Defensin In Ticks: Molecular Characterization, Expression, And Role In Innate Immunity, Daniel E. Sonenshine, Wayne L. Hynes, Shane M. Ceraul, Michelle Todd
Biological Sciences Faculty Publications
No abstract provided.