Life Sciences Commons^™

Integrated Analysis Of Microrna And Mrna Transcriptome Reveals The Molecular Mechanism Of Solanum Lycopersicum Response To Bemisia Tabaci And Tomato Chlorosis Virus, Hao Yue, Li-Ping Huang, Ding-Li-Hui Lu, Zhan-Hong Zhang, Zhuo Zhang, De-Yong Zhang, Li-Min Zheng, Yang Gao, Xin-Qiu Tan, Xuguo Zhou, Xiao-Bin Shi, Yong Liu

Entomology Faculty Publications

Tomato chlorosis virus (ToCV), is one of the most devastating cultivated tomato viruses, seriously threatened the growth of crops worldwide. As the vector of ToCV, the whitefly Bemisia tabaci Mediterranean (MED) is mainly responsible for the rapid spread of ToCV. The current understanding of tomato plant responses to this virus and B. tabaci is very limited. To understand the molecular mechanism of the interaction between tomato, ToCV and B. tabaci, we adopted a next-generation sequencing approach to decipher miRNAs and mRNAs that are differentially expressed under the infection of B. tabaci and ToCV in tomato plants. Our data revealed …

De Novo Whole Genome Assembly Of The Swede Midge (Contarinia Nasturtii), A Specialist Of Brassicaceae, Using Linked-Read Sequencing, Boyd A. Mori, Cathy Coutu, Yolanda H. Chen, Erin O. Campbell, Julian R. Dupuis, Martin A. Erlandson, Dwayne D. Hegedus

Entomology Faculty Publications

The swede midge, Contarinia nasturtii, is a cecidomyiid fly that feeds specifically on plants within the Brassicaceae. Plants in this family employ a glucosinolate-myrosinase defense system, which can be highly toxic to non-specialist feeders. Feeding by C. nasturtii larvae induces gall formation, which can cause substantial yield losses thus making it a significant agricultural pest. A lack of genomic resources, in particular a reference genome, has limited deciphering the mechanisms underlying glucosinolate tolerance in C. nasturtii, which is of particular importance for managing this species. Here, we present an annotated, scaffolded reference genome of C. nasturtii using linked-read …

Genome-Wide Analysis Of Atp-Binding Cassette (Abc) Transporters In The Sweetpotato Whitefly, Bemisia Tabaci, Lixia Tian, Tianxue Song, Rongjun He, Yang Zeng, Wen Xie, Qingjun Wu, Shaoli Wang, Xuguo Zhou, Youjun Zhang

Entomology Faculty Publications

Background: ABC transporter superfamily is one of the largest and ubiquitous groups of proteins. Because of their role in detoxification, insect ABC transporters have gained more attention in recent years. In this study, we annotated ABC transporters from a newly sequenced sweetpotato whitefly genome. Bemisia tabaci Q biotype is an emerging global invasive species that has caused extensive damages to field crops as well as ornamental plants.

Results: A total of 55 ABC transporters containing all eight described subfamilies (A to H) were identified in the B. tabaci Q genome, including 8 ABCAs, 3 ABCBs, 6 ABCCs, 2 ABCDs, 1 …

Transcriptomic Response Of Wolf Spider, Pardosa Pseudoannulata, To Transgenic Rice Expressing Bacillus Thuringiensis Cry1ab Protein, Juan Wang, Yuande Peng, Kaifu Xiao, Baoyang Wei, Jilin Hu, Zhi Wang, Qisheng Song, Xuguo Zhou

Entomology Faculty Publications

Background: Bacillum thuringiensis (Bt) toxin produced in Cry1-expressing genetically modified rice (Bt rice) is highly effective to control lepidopteran pests, which reduces the needs for synthetic insecticides. Non-target organisms can be exposed to Bt toxins through direct feeding or trophic interactions in the field. The wolf spider Pardosa pseudoannulata, one of the dominant predators in South China, plays a crucial role in the rice agroecosystem. In this study, we investigated transcriptome responses of the 5th instar spiders fed on preys maintained on Bt- and non-Bt rice.

Results: Comparative transcriptome analysis resulted in 136 differentially expressed genes (DEGs) …

Genome-Wide Transcriptomic And Proteomic Analyses Of Bollworm-Infested Developing Cotton Bolls Revealed The Genes And Pathways Involved In The Insect Pest Defence Mechanism, Saravanan Kumar, Kanakachari Mogilicherla, Dhandapani Gurusamy, Krishan Kumar, Prabhakaran Narayanasamy, Padmalatha Kethireddy Venkata, Amolkumar Solanke, Savita Gamanagatti, Vamadevaiah Hiremath, Ishwarappa S. Katageri, Sadhu Leelavathi, Polumetla Ananda Kumar, Vanga Siva Reddy

Entomology Faculty Publications

Cotton bollworm, Helicoverpa armigera, is a major insect pest that feeds on cotton bolls causing extensive damage leading to crop and productivity loss. In spite of such a major impact, cotton plant response to bollworm infection is yet to be witnessed. In this context, we have studied the genome-wide response of cotton bolls infested with bollworm using transcriptomic and proteomic approaches. Further, we have validated this data using semi-quantitative real-time PCR. Comparative analyses have revealed that 39% of the transcriptome and 35% of the proteome were differentially regulated during bollworm infestation. Around 36% of significantly regulated transcripts and 45% …

Stably Expressed Housekeeping Genes Across Developmental Stages In The Two-Spotted Spider Mite, Tetranychus Urticae, Chunxiao Yang, Huipeng Pan, Yong Liu, Xuguo Zhou

Entomology Faculty Publications

Quantitative real-time PCR (qRT-PCR) is a reliable and reproducible technique for measuring mRNA expression. To facilitate gene expression studies and obtain more accurate qRT-PCR analysis, normalization relative to stable housekeeping genes is mandatory. In this study, ten housekeeping genes, including beta-actin (Actin), elongation factor 1 α (EF1A), glyceralde hyde-3-phosphate dehydrogenase (GAPDH), ribosomal protein L13 (RPL13), ribosomal protein 49 (RP49), α-tubulin (Tubulin), vacuolar-type H+-ATPase (v-ATPase), succinate dehydrogenase subunit A (SDHA) , 28S ribosomal RNA (28S), and 18S ribosomal RNA (18S) from …

Identification And Developmental Profiling Of Micrornas In Diamondback Moth, Plutellaxylostella (L.), Pei Liang, Bing Feng, Xuguo Zhou, Xiwu Gao

Entomology Faculty Publications

MicroRNAs (miRNAs) are a group of small RNAs involved in various biological processes through negative regulation of mRNAs at the post-transcriptional level. Although miRNA profiles have been documented in over two dozen insect species, few are agricultural pests. In this study, both conserved and novel miRNAs in the diamondback moth, Plutella xylostella L., a devastating insect pest of cruciferous crops worldwide, were documented. High-throughput sequencing of a small RNA library constructed from a mixed life stages of P. xylostella, including eggs, 1st to 4th (last) instar larvae, pupae and adults, identified 384 miRNAs, of which 174 were P. xylostella specific. …

Characterization Of Head Transcriptome And Analysis Of Gene Expression Involved In Caste Differentiation And Aggression In Odontotermes Formosanus (Shiraki), Qiuying Huang, Pengdong Sun, Xuguo Zhou, Chaoliang Lei

Entomology Faculty Publications

Background

The subterranean termite Odontotermes formosanus (Shiraki) is a serious insect pest of trees and dams in China. To date, very little is known about genomic or transcriptomic data for caste differentiation and aggression in O. formosanus. Hence, studies on transcriptome and gene expression profiling are helpful to better understand molecular basis underlying caste differentiation and aggressive behavior in O. formosanus.

Methodology and Principal Findings

Using the Illumina sequencing, we obtained more than 57 million sequencing reads derived from the heads of O. formosanus. These reads were assembled into 116,885 unique sequences (mean size = 536 bp). …

Pyrosequencing The Bemisia Tabaci Transcriptome Reveals A Highly Diverse Bacterial Community And A Robust System For Insecticide Resistance, Wen Xie, Qing-Shu Meng, Qing-Jun Wu, Shao-Li Wang, Xin Yang, Ni-Na Yang, Ru-Mei Li, Xiao-Guo Jiao, Hui-Peng Pan, Bai-Ming Liu, Qi Su, Bao-Yun Xu, Song-Nian Hu, Xuguo Zhou, You-Jun Zhang

Entomology Faculty Publications

BACKGROUND: Bemisia tabaci (Gennadius) is a phloem-feeding insect poised to become one of the major insect pests in open field and greenhouse production systems throughout the world. The high level of resistance to insecticides is a main factor that hinders continued use of insecticides for suppression of B. tabaci. Despite its prevalence, little is known about B. tabaci at the genome level. To fill this gap, an invasive B. tabaci B biotype was subjected to pyrosequencing-based transcriptome analysis to identify genes and gene networks putatively involved in various physiological and toxicological processes.

METHODOLOGY AND PRINCIPAL FINDINGS: Using Roche 454 pyrosequencing, …