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Synchrotron X-Ray Visualisation Of Ice Formation In Insects During Lethal And Non-Lethal Freezing, Brent J. Sinclair, Allen G. Gibbs, Wah Keat Lee, Arun Rajamohan, Stephen P. Roberts, John J. Socha
Synchrotron X-Ray Visualisation Of Ice Formation In Insects During Lethal And Non-Lethal Freezing, Brent J. Sinclair, Allen G. Gibbs, Wah Keat Lee, Arun Rajamohan, Stephen P. Roberts, John J. Socha
Biological Sciences Faculty Research & Creative Works
Although the Biochemical Correlates of Freeze Tolerance in Insects Are Becoming Well-Known, the Process of Ice Formation in Vivo is Subject to Speculation. We Used Synchrotron X-Rays to Directly Visualize Real-Time Ice Formation at 3.3 Hz in Intact Insects. We Observed Freezing in Diapausing 3rd Instar Larvae of Chymomyza Amoena (Diptera: Drosophilidae), Which Survive Freezing If It Occurs above 214uC, and non-Diapausing 3rd Instar Larvae of C. Amoena and Drosophila Melanogaster (Diptera: Drosophilidae), Neither of Which Survive Freezing. Freezing Was Readily Observed in All Larvae, and on One Occasion the Gut Was Seen to Freeze Separately from the Haemocoel. There …