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Changes In Thermotolerance And Hsp70 Expression With Domestication In Drosophila Melanogaster, R. A. Krebs, S. (Stephen) P. Roberts, B. R. Bettencourt, M. E. Feder

Biological Sciences Faculty Research & Creative Works

To Examine How the Duration of Laboratory Domestication May Affect Drosophila Stocks Used in Studies of Thermotolerance, We Measured Expression of the Inducible Heat-Shock Protein Hsp70 and Survival after Heat Shock in D. Melanogaster Strains Recently Collected from Nature and Maintained in Laboratory Culture for Up to 50 or More Generations. after an Initial Increase in Both Hsp70 Expression and Thermotolerance Immediately after Transfer to Laboratory Medium, Both Traits Remained Fairly Constant over Time and Variation among Strains Persisted through Laboratory Domestication. Furthermore, Variation in Heat Tolerance and Hsp70 Expression Did Not Correlate with the Length of Time Populations Evolved …