Life Sciences Commons^™

Broadband In Nebraska: Current Landscape And Recommendations, Nebraska Information Technology Commission, Nebraska Broadband Initiative, Nebraska Public Service Commission, University Of Nebraska-Lincoln, Nitc Community Council, Nebraska Department Of Economic Development, Aim

Rural Futures Institute: Publications

N ebraska’s broadband vision is that residents, businesses, government entities, commu-nity partners, and visitors have access to affordable broadband service and have the necessary skills to effectively utilize broadband technologies.

Objectives

To increase economic development opportunities, create good-paying jobs, at-tract and retain population, overcome the barriers of distance, and enhance qual-ity of life in Nebraska by stimulating the continuing deployment of broadband technologies which meet the need for increasing connection speeds.

To increase digital literacy and the widespread adoption of broadband technolo-gies in business, agriculture, health care, education, government and by individu-al Nebraskans.

Goals

The following goals and targets help …

Long-Acting Antituberculous Therapeutic Nanoparticles Target Macrophage Endosomes, Benson J. Edagwa, Dongwei Guo, Pavan Puligujja, Han Chen, Joellyn Mcmillan, Xinming Liu, Howard Gendelman, Prabagaran Narayanasamy

Nebraska Center for Biotechnology: Faculty and Staff Publications

Eradication of Mycobacterium tuberculosis (MTB) infection requires daily administration of combinations of rifampin (RIF), isoniazid [isonicotinylhydrazine (INH)], pyrazinamide, and ethambutol, among other drug therapies. To facilitate and optimize MTB therapeutic selections, a mononuclear phagocyte (MP; monocyte, macrophage, and dendritic cell)-targeted drug delivery strategy was developed. Long-acting nanoformulations of RIF and an INH derivative, pentenyl- INH (INHP), were prepared, and their physicochemical properties were evaluated. This included the evaluation of MP particle uptake and retention, cell viability, and antimicrobial efficacy. Drug levels reached 6 μg/106 cells in human monocyte-derived macrophages (MDMs) for nanoparticle treatments compared with 0.1 μg/106 cells for native …

Feedlot Manure Management Considerations Including Anaerobic Digestion Potential And Mineral Retention, Andrea K. Watson

Department of Animal Science: Dissertations, Theses, and Student Research

Three anaerobic digestion trials were conducted to evaluate methane production from feedlot manure. As organic matter content of manure decreased, degradation of manure and methane production also linearly decreased (P ≤ 0.02). Quality, or organic matter content of manure, had a greater impact on anaerobic digestion than diet (with and without distillers grains) fed to cattle. Minerals are concentrated in effluent removed from anaerobic digesters.

Knowing mineral retention within cattle allows for more accurate calculation of mineral excretion in manure. Three serial slaughter trials measured mineral retention in growing and finishing cattle. During a growing phase, beef cattle gaining …

The Figure Of Bitu-Man, Paul Royster

UNL Libraries: Faculty Publications

The ominously imposing figure in the above illustration is “the Syncrude bird deterrent device, locally known as ‘Bitu-man’.” He stands (or stood—the original image was from the late 1970s) in the tailings pond of the Syncrude Canada Ltd. oil sands mining operation along the Athabasca River near Fort McMurray in northeastern Alberta. That operation surface mines (i.e., strip mines) bitumen-impregnated sand, processes it with hot water and steam, and discharges the liquid effluent or “tailings” into a pond covering roughly 3000 hectares (11 square miles). (Google Map coordinates: 56.9°N, -111.3° W). Residue bitumen collects on the surface and poses a …

Virus-Host Mucosal Interactions During Early Siv Rectal Transmission, Wuxun Lu, Fangrui Ma, Alexander Churbanov, Yanmin Wan, Yue Li, Guobin Kang, Zhe Yuan, Dong Wang, Chi Zhang, Jianqing Xu, Mark Lewis, Qingsheng Li

Nebraska Center for Biotechnology: Faculty and Staff Publications

To deepen our understanding of early rectal transmission of HIV-1, we studied virus-host interactions in the rectal mucosa using simian immunodeficiency virus (SIV)-Indian rhesus macaque model and mRNA deep sequencing. We found that rectal mucosa actively responded to SIV as early as 3 days post-rectal inoculation (dpi) and mobilized more robust responses at 6 and 10 dpi. Our results suggests that the failure of the host to contain virus replication at the portal of entry is attributable to both a high-level expression of lymphocyte chemoattractant, proinflammatory and immune activation genes, which can recruit and activate viral susceptible target cells into …

A Case Study Of Changing Cropping Diversity And Agricultural Risk In The Doulthabad Mandal Of Telangana State In India, Srikanth Kondabolu

School of Natural Resources: Dissertations, Theses, and Student Research

The Doulthabad^[1] Mandal is a hot semi-arid agro-ecological sub-region (Rao et al. 2006) located in Mahbubnagar District on the North Telangana Plateau in the state of Telangana. Agriculture is the main occupation in this region, which is populated mostly with small and marginal farmers. This study uses the time period from 1971 to 2004 to study the variation in cropping pattern diversity and distribution of rainfall during the monsoons and understand the implications on production risk in agriculture. Quantitative methods were used in determining the changes in rainfall while qualitative methods were used to study cropping system changes. The …

Infiltration And Runoff Parameters For Tilled And No-Till Row Crops, Andrew J. Volkmer

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Infiltration and runoff are hydrologic processes that effect the amount of water available to plants, for groundwater recharge, and for stream flow. No-till planting (NT) is a management practice used to reduce soil erosion, increase water infiltration, and reduce soil water evaporation, and can have great impact on infiltration and runoff. An investigation was conducted to determine the impact of NT on infiltration and runoff when compared to tilled conditions.

Runoff and precipitation data was gathered from sites at Fillmore County, NE, Phelps County, NE, the USDA-ARS North Appalachian Experimental Watersheds (NAEW) near Coshocton, OH, and the Lennoxville Research Station …

Feeding Condensed Distillers Solubles To Feedlot Finishing Steers And The Effects Of Feed Additives In Adaptation Diets, Marie E. Harris

Department of Animal Science: Dissertations, Theses, and Student Research

By-products from the dry-milling ethanol process can be used in cattle diets to replace corn. There is a significant interaction between corn processing methods as CCDS concentration increases in the diet. Improvements are observed for SFC in final BW, ADG, and G:F over DRC. An experiment was conducted to determine if greater concentrations of CCDS could be fed in SFC based diets and maintain or improve performance. Performance and carcass characteristics were evaluated with increasing concentrations of CCDS at 0, 9, 18, 27, or 36% in place of SFC in feedlot finishing diets. As CCDS concentration increased, DMI decreased quadratically. …

Economic Impacts Of Increased Corporate Average Fuel Economy (Cafe) Standards, Ann K. Hunter-Pirtle

Department of Agricultural Economics: Dissertations, Theses, and Student Research

The Renewable Fuel Standard (RFS) mandates that U.S. transportation fuel producers blend specific volumes of ethanol and other biofuels with fossil fuels to spur U.S. biofuel production and to minimize foreign oil imports. Ethanol is more corrosive to auto engines than gasoline, and although vehicles manufactured since 2001 are approved to use up to a 15% ethanol blend (E15) (Naylor & Falcon, 2011), E10 is much more widely available. Ethanol producers therefore face a so-called blend wall at 10 percent—a maximum amount of ethanol that is usable domestically based on the demand for gasoline.

Meanwhile, gasoline demand in the U.S. …

Production Of Recombinant Human Coagulation Factor Ix By Transgenic Pig, Weijie Xu

Department of Chemical and Biomolecular Engineering: Theses and Student Research

Hemophilia B is the congenital bleeding disorder caused by deficiency in functional coagulation factor IX (FIX) and about 28,000 patients worldwide in 2012. And current treatment is restricted to protein-replacement therapy, which required FIX concentrates for patients’ life-time. Approximately 1 billion units FIX were consumed in 2012. However, still about 70-80% patients, mostly in developing countries, received inadequate or no treatment because of the unavailable and/or unaffordable FIX concentrates. Considering safety reasons, e.g. transmission of blood-borne diseases, the recombinant human FIX (rFIX) is recommended other than the plasma-derived FIX. However, only one rFIX is currently available on the market. The …

Informing Flood Plain Wetland Restoration Using Amphibian Monitoring, Ashley Vanderham

School of Natural Resources: Dissertations, Theses, and Student Research

Wetlands are among the most important and complex ecosystems in the world. They contribute to nutrient cycling, the hydrologic cycle, and provide critical habitat for many plants, fish, and wildlife. Channelization of Missouri River resulted in the loss of many floodplain wetlands. Despite ongoing restoration efforts, there are few ecologically-based performance guidelines, and managers need methods to quantify and assess the success of restored riverine wetland systems.

In 2008 a multi-institutional herpetofauna monitoring project, funded by the U.S. Army Corps of Engineers, was initiated in four states (Kansas, Missouri, Iowa, and Nebraska). The main goal of the project is to …

Juvenile Green Sturgeon (Acipenser Medirostris) And White Sturgeon (Acipenser Transmontanus) Behavior Near Water-Diversion Fish Screens: Experiments In A Laboratory Swimming Fume, Jamilynn B. Poletto, Dennis E. Cocherell, Natalie Ho, Joseph J. Cech Jr., A. Peter Klimley, Nann A. Fangue

School of Natural Resources: Faculty Publications

Water diversions that extract fresh water for urban, industrial, and agricultural uses, as well as export to southern California, are prevalent throughout the Sacramento–San Joaquin watershed. Many water diversions are fitted with fish-exclusion screens designed to prevent fish from entrainment (i.e., being drawn in). The impact of fish screens on the behavior of migrating juvenile fishes remains largely unknown, especially for threatened species such as sturgeon. We placed individual juvenile green (Acipenser medirostris) or white (Acipenser transmontanus) sturgeon in a laboratory swimming flume in the presence of standard fish screens (2 mm bar spacing) at two …

Characterization Of Genetically Modified High Biomass Producing Tobacco Plant, Pankaj Singh Kuhar

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Global warming and peak oil has clouded our energy security. In light of this situation, bioethanol as emerged as one of the most amenable solutions to the problem. However bioethanol has its own shortcomings and transgenics seem imperative to exploit its full potential. A high biomass producing line in transgenic tobacco (Nicotiana tabacum cv. Xanthi) was identified during a routine genetic transformation, termed giant recombinant (GR). To characterize the phenotype of the giant line, growth rate and lignocellulosic composition was analyzed relative to the non-transgenic control line. The GR line accounted for 240% more biomass than the untransformed line …

Feeding Alkaline Treated And Processed Crop Residue To Feedlot Cattle, Sarah J. Peterson

Department of Animal Science: Dissertations, Theses, and Student Research

Chemical treatment and decreased particle size are methods used to improve digestibility and utilization of the available nutrients in low quality forages. Previous research has indicated that chemically treated corn residue can take the place of corn when included in finishing rations containing distillers grains. Also, decreasing particle size utilizing methods such as pelleting has been shown to improve DMI and ADG. However, limited research has been completed on use of chemical treatment and pelleting in growing and receiving rations. Also, an ideal distillers inclusion has not yet been identified when including alkaline treated stalks in finishing rations. Therefore, a …

Determining Forage Intake Of Nursing Calves Grazing Native Sandhills Rangeland, Jennifer A. Walker

Department of Animal Science: Dissertations, Theses, and Student Research

Effective management of grazing lands is important for longevity and productivity. Accurate calculations of stocking rates per animal unit will aid in long term sustainability. Calculating stocking rates for cattle can prove challenging for cows and nursing calves. Limited research has been conducted to determine forage intake of the nursing calf pre-weaning. The objective of Trial 1 was to determine nursing calf forage intake over time until weaning. Trial 1 used 8 crossbred cow-calf pairs in experiment 1 and 12 pairs in experiment 2. Pairs were separated into two groups rotating between grazing upland range or housed in individual pens …

Response Of Large River Fishes To A Prolonged High Water Event In The Missouri River, Nebraska, Nicholas Paul Hogberg

School of Natural Resources: Dissertations, Theses, and Student Research

Regulation and modification of large rivers to accommodate human uses have been a root cause of freshwater biodiversity declines. The Missouri River is among the most drastically-altered large river systems in North America, with a series of mainstem impoundments in the upper watershed altering flow characteristics downstream, and channelization throughout the lower river homogenizing instream habitat and reducing off-channel habitat. Precipitation events during the winter and spring 2010-2011 caused flooding of the greatest magnitude and duration since reservoir completion. The large magnitude and long duration of this flood made it unlike any flood in recent history and provided a unique …

Investigating Antibiotic Resistance Levels Of Salmonella Internalized In Lettuce Leaves, Jason B. Thomas

McNair Scholars Research Journal

Contamination of food crops by the human pathogen Salmonella is a food safety threat worldwide. Though using treated wastewater for irrigation is a sustainable practice, it may introduce trace levels of Salmonella that may contaminate food crops. Salmonella could develop resistance to antibiotics present in wastewater. The overall goal of the project is to increase the understanding of the public health risk associated with the use of treated wastewater to irrigate food crops. The objective of this particular study is to determine the antibiotic resistance level of Salmonella internalized in lettuce leaves. In this experiment, thirty-six plants of the lettuce …

Starch-Pectin Matrices For Encapsulation Of Ascorbic Acid, Yiwei Liu

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Starch and pectin are two food-grade carbohydrates widely utilized in the food industry. Starch and pectin polymers have been investigated in encapsulating functional food ingredients and in pharmaceutical applications. Resistance to enzyme hydrolysis, differential solubilities, depending on the pH, and the ability to ‘protect’ unstable molecules are considered some of the beneficial properties of starch and pectin polymers, for encapsulation applications. Food ingredients could be delivered in a controlled manner to a specific target by encapsulating in micro-scale particles, i. e., microencapsulation. Two studies were conducted to investigate the ability of selected starch-pectin blends in microencapsulating ascorbic acid (vitamin C) …

Uncertainties In Life Cycle Greenhouse Gas Emissions From U.S. Beef Cattle, Quentin M. Dudley, Adam Liska, Andrea K. Watson, Galen E. Erickson

Adam Liska Papers

Beef cattle feedlots are estimated to contribute 26% of U.S. agricultural greenhouse gas (GHG) emissions, and future climate change policy could target reducing these emissions. Life cycle assessment (LCA) of GHG emissions from U.S. grain-fed beef cattle was conducted based on industry statistics and previous studies to identify the main sources of uncertainty in these estimations. Uncertainty associated with GHG emissions from indirect land use change, pasture soil emissions (e.g. soil carbon sequestration), enteric fermentation from cattle on pasture, and methane emissions from feedlot manure, respectively, contributed the most variability to life cycle GHG emissions from beef production. Feeding of …

Integrating Mammalian Hazards With Management At U.S. Civil Airports: A Case Study., Kristin M. Biondi, Jerrold L. Belant, James A. Martin, Travis L. Devault, Guiming Wang

USDA Wildlife Services: Staff Publications

Wildlife incidents with U.S. civil aircraft cost an estimated $1.4 billion from 1990 to 2010, with mammals 5 times more likely to cause damage than other wildlife. We surveyed 2 general aviation (GA) airports and 6 Part-139 certificated (i.e., certified) airports to assess efficacy of management practices for mammalian species hazardous to aircraft. We obtained information on mammalian species present on airport grounds, types and estimated effectiveness of management techniques, and effort spent on wildlife management. We evaluated management techniques relative to aircraft–wildlife collisions (i.e., incident) frequencies taken from Federal Aviation Administration’s (FAA) National Wildlife Strike Database and species hazard …

Variedades De Sorgos Bmr: Una Alternativa Para Producir Forraje, Instituto Nacional De Innovación Y Transferencia En Tecnología Agropecuaria (Inta-Costa Rica), Roberto Tinoco Mora

INTSORMIL Scientific Publications

Nervadura de color café en variedad de sorgo BMR.

Durante el año 2010, el Instituto Nacional de Innovación y Transferencia en Tecnología Agropecuaria (INTA-Costa Rica), conjuntamente con el Programa Colaborativo y de Apoyo a la Investigación del Sorgo, el Mijo y otros Granos (INTSORMIL, CRSP) ubicado en El Salvador. elaboraron un proyecto de investigación para evaluar la adaptación, producción y valor nutritivo de una colección de 15 variedades de sorgo (Sorghum bicolor) para alimentación animal. Las variedades disponían del gen recesivo denominado BMR, siglas en inglés de "Brown mid rib" que significan "vena café" (Figura 1).

Este gen tiene la …

Information In Biological Systems And The Fluctuation Theorem, Yaşar Demirel

Department of Chemical and Biomolecular Engineering: Faculty Publications

Some critical trends in information theory, its role in living systems and utilization in fluctuation theory are discussed. The mutual information of thermodynamic coupling is incorporated into the generalized fluctuation theorem by using information theory and nonequilibrium thermodynamics. Thermodynamically coupled dissipative structures in living systems are capable of degrading more energy, and processing complex information through developmental and environmental constraints. The generalized fluctuation theorem can quantify the hysteresis observed in the amount of the irreversible work in nonequilibrium regimes in the presence of information and thermodynamic coupling.

Physiological And Transcriptional Memory In Guard Cells During Repetitive Dehydration Stress, Laetitia Virlouvet, Michael E. Fromm

Nebraska Center for Biotechnology: Faculty and Staff Publications

Arabidopsis plants subjected to a daily dehydration stress and watered recovery cycle display physiological and transcriptional stress memory. Previously stressed plants have stomatal apertures that remain partially closed during a watered recovery period, facilitating reduced transpiration during a subsequent dehydration stress. Guard cells (GCs) display transcriptional memory that is similar to that in leaf tissues for some genes, but display GC-specific transcriptional memory for other genes. The rate-limiting abscisic acid (ABA) biosynthetic genes NINE-CIS-EPOXYCAROTENOID DIOXYGENASE 3 (NCED3) and ALDEHYDE OXIDASE 3 (AAO3) are expressed at much higher levels in GCs, particularly during the watered recovery interval, relative to their low …

The Pseudomonas Syringae Type Iii Effector Hopd1 Suppresses Effector-Triggered Immunity, Localizes To The Endoplasmic Reticulum, And Targets The Arabidopsis Transcription Factor Ntl9, Anna Block, Tania Y. Toruno, Christian G. Elowsky, Chi Zhang, Jens Steinbrenner, Jim Beynon, James R. Alfano

Nebraska Center for Biotechnology: Faculty and Staff Publications

Pseudomonas syringae type III effectors are known to suppress plant immunity to promote bacterial virulence. However, the activities and targets of these effectors are not well understood. We used genetic, molecular, and cell biology methods to characterize the activities, localization, and target of the HopD1 type III effector in Arabidopsis. HopD1 contributes to P. syringae virulence in Arabidopsis and reduces effector-triggered immunity (ETI) responses but not pathogen-associated molecular pattern-triggered immunity (PTI) responses. Plants expressing HopD1 supported increased growth of ETI-inducing P. syringae strains compared with wild-type Arabidopsis. We show that HopD1 interacts with the membrane-tethered Arabidopsis transcription factor NTL9 and …

Fate Of Antimicrobials And Antimicrobial Resistance Genes In Simulated Swine Manure Storage, Stacey R. Joy, Xu Li, Daniel D. Snow, John Gilley, Bryan L. Woodbury, Shannon L. Bartelt-Hunt

School of Natural Resources: Faculty Publications

The behavior of three antibiotics (bacitracin, chlortetracycline, and tylosin) and two classes of antibiotic resistance genes (ARGs), tet and erm, were monitored in swine manure slurry under anaerobic conditions. First-order decay rates were determined for each antibiotic with half-lives ranging from1 day (chlortetracycline) to 10 days (tylosin). ARGs were monitored in the swine manure slurry, and losses of approximately 1 to 3 orders of magnitude in relative abundance were observed during the 40 day storage period. First-order degradation profiles were observed for chlortetracycline and its corresponding resistance genes, tet(X) and tet(Q). Tylosin was degraded to approximately 10% …