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Full-Text Articles in Life Sciences
Metabolic Engineering Of A Thermophilic Bacterium To Produce Ethanol At High Yield, A. Joe Shaw, Kara K. Podkaminer, Sunil G. Desai, John S. Bardsley, Stephen R. Rogers, Philip G. Thorne, David A. Hogsett, Lee R. Lynd
Metabolic Engineering Of A Thermophilic Bacterium To Produce Ethanol At High Yield, A. Joe Shaw, Kara K. Podkaminer, Sunil G. Desai, John S. Bardsley, Stephen R. Rogers, Philip G. Thorne, David A. Hogsett, Lee R. Lynd
Dartmouth Scholarship
We report engineering Thermoanaerobacterium saccharolyticum, a thermophilic anaerobic bacterium that ferments xylan and biomass-derived sugars, to produce ethanol at high yield. Knockout of genes involved in organic acid formation (acetate kinase, phosphate acetyltransferase, and L-lactate dehydrogenase) resulted in a strain able to produce ethanol as the only detectable organic product and substantial changes in electron flow relative to the wild type. Ethanol formation in the engineered strain (ALK2) utilizes pyruvate:ferredoxin oxidoreductase with electrons transferred from ferredoxin to NAD(P), a pathway different from that in previously described microbes with a homoethanol fermentation. The homoethanologenic phenotype was stable for >150 generations …
Physical And Chemical Properties Of Corn Distillers Wet Grains, Kurt A. Rosentrater, R. Michael Lehman
Physical And Chemical Properties Of Corn Distillers Wet Grains, Kurt A. Rosentrater, R. Michael Lehman
Kurt A. Rosentrater
The production of corn-based ethanol in the United States is dramatically increasing, and consequently, so is the quantity of generated coproducts, all of which are currently fed to livestock. Distillers Wet Grain (DWG) is one of these coproduct feeds. Many studies have investigated the nutritional properties of DWG and the response of livestock (primarily beef and dairy) to inclusion of these ingredients in their diets. To date, physical properties have been largely ignored, but they are needed for the design of storage facilities and equipment. The objective of this research was to quantify physical and chemical property values for typical …