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Full-Text Articles in Life Sciences
2013 Annual Report Of Uvm Extension And The Vermont Agricultural Experiment Station, Uvm Extension And Vermont Agricultural Experiment Station
2013 Annual Report Of Uvm Extension And The Vermont Agricultural Experiment Station, Uvm Extension And Vermont Agricultural Experiment Station
Annual Reports
2013 Annual report of outreach and research from UVM Extension and Vermont Agricultural Experiment Station.
Regulation Of Β-Casein Gene Expression By Octamer Transcription Factors And Utilization Of Β-Casein Gene Promoter To Produce Recombinant Human Proinsulin In The Transgenic Milk, Xi Qian
Graduate College Dissertations and Theses
β-Casein is a major milk protein, which is synthesized in mammary alveolar secretory epithelial cells (MECs) upon the stimulation of lactogenic hormones, mainly prolactin and glucocorticoids (HP). Previous studies revealed that the proximal promoter (-258 bp to +7 bp) of the β-casein gene is sufficient for induction of the promoter activity by HP. This proximal region contains the binding sites for the signal transducer and activator of transcription 5 (STAT5), glucocorticoid receptor (GR), and octamer transcription factors (Oct). STAT5 and GR are essential downstream mediators of prolactin and glucocorticoid signaling, respectively. This study investigated the functions of Oct-1 and Oct-2 …
Understanding And Improving Microbial Biofuel Tolerance As A Result Of Efflux Pump Expression Through Genetic Engineering And Mathematical Modeling, William James Turner
Understanding And Improving Microbial Biofuel Tolerance As A Result Of Efflux Pump Expression Through Genetic Engineering And Mathematical Modeling, William James Turner
Graduate College Dissertations and Theses
Recent advances in synthetic biology have enabled the construction of non-native metabolic pathways for production of next-generation biofuels in microbes. One such biofuel is the jet-fuel precursor α-pinene, which can be processed into high-energy pinene dimers. However, accumulation of toxic biofuels in the growth medium limits the possible fuel yield. Overexpression of transporter proteins such as efflux pumps can increase tolerance to biofuels by pumping them out of the cell, thus improving fuel yields. However, too many efflux pumps can compromise the cell as well, creating a trade-off between biofuel toxicity and pump toxicity. In this work we improve the …