Life Sciences Commons^™

Moving Forward: Preventing Water Shortage For Nevada, Sandra Blandon, Brianna Lyon

Anthropology 100: Feast and Famine In a Global World Poster Assignment

Southern Nevada is located in the arid Mojave Desert, which averages about 4 inches of rain each year. Southern Nevada gets about 90% of its water supply from the Colorado River. Seven western states and Mexico share the river. This means that The Colorado River provides water to 25 million people.

Isolation, Characterization, And Genome Sequence Of The First Representative Of A Novel Class Within The Chloroflexi That Is Abundant In Some U.S. Great Basin Hot Springs And May Play Important Roles In N And C Cycling, Jeremy A. Dodsworth, Senthil K. Murugapiran, Jonathan Gevorkian, James Han, Tanja Woyke, Susan M. Lucas, Sam Pitluck, Len Pennacchio, Lynne Goodwin, Brian P. Hedlund

Undergraduate Research Opportunities Program (UROP)

A thermophilic, facultatively microaerophilic, heterotrophic bacterium, designated strain JAD2, was isolated from sediments of Great Boiling Spring (GBS), an ~80oC, circumneutral hot spring in the Great Basin GB). The strain grew anaerobically on yeast extract or peptone with an optimal growth temperature of 70-75oC. Growth was stimulated by addition of 0.01 atm O2 to the culture vessel headspace, but was inhibited by higher concentrations (0.2 atm). Cells of JAD2 formed non-motile filaments ranging from 10 to >300 μm in length, which typically decreased in length during stationary phase. 16S rRNA gene-targeted pyrotag sequencing and clone library data suggest that close …

Magnetosome Genes In The Gammaproteobacterium Strain Bw-2, Lucero Rivera, Denis Trubitsyn, Dennis A. Bazylinski

Undergraduate Research Opportunities Program (UROP)

Magnetotactic bacteria (MTB) biomineralize intracellular nanometer-sized, magnetic crystals surrounded by a lipid bilayer membrane known as magnetosomes. These crystals, which consist of magnetite (Fe3O4) or greigite (Fe3S4), causes the cell to align along the geomagnetic field lines as they swim, a phenomenon known as magnetotaxis. Strain BW-2 is a magnetite-producing magnetotactic bacterium isolated from Badwater Basin, Death Valley National Park (California) and is one of only two species of MTB that are known to phylogenetically belong to the Gammaproteobacteria class of the Proteobacteria phylum. The biomineralization of magnetite in magnetotactic bacteria is mediated by a series of genes that include …

A Spatial And Temporal Analysis Of Microbial Communities In Great Boiling Spring, Nevada, U.S.A., Jessica K. Guy, Joseph P. Peacock, Jeremy A. Dodsworth, Tanja Woyke, Tijana G. Del Rio, Brian P. Hedlund

Undergraduate Research Opportunities Program (UROP)

Great Boiling Spring (GBS) is a large, circumneutral, long residence time geothermal spring in the US Great Basin. Twelve samples were taken from four different sediment sites and the planktonic community in the bulk water of GBS on up to four different dates. Microbial community composition and diversity was assessed by using a barcoded, improved universal primer set targeting the V8 portion of the 16S rRNA gene and PCR. Over 200,000 products were sequenced using the Roche 454 GS FLX Titanium System. Sediment and planktonic microbial communities were distinct with very little overlap, regardless of the sampling location or temperature. …

Biological Effects On Serpentinite Weathering, Mary H. Evert, Julie Baumeister, Elisabeth Hausrath

Undergraduate Research Opportunities Program (UROP)

Serpentinites, perhaps more than any other rock type, control the composition and evolution of the development of the surrounding ecosystems. The bulk chemistry of serpentinite rocks, high in Mg and trace elements, and low in nutrients such as Ca, K, P, and N, causes an extreme and stressful environment for ecosystems. However, the role that those serpentine ecosystems play in development of serpentine soils has not been examined.

Due to the unusual chemistry of serpentine soils, serpentine ecosystems have deeper and better-developed root systems than other ecosystems. The rhizosphere of serpentine systems, documented to produce abundant organic acids and siderophores, …

The Use Of Chloramines To Eradicate Quagga Mussel Larvae, Trea Lacroix, Kumud Acharya

Undergraduate Research Opportunities Program (UROP)

Quagga Mussels, Dressenia bugensis, are a growing problem in the western United States, particularly in their ability to infest underwater infrastructures and clog water intake pipes and screens of power and treatment plants. Chlorine has been found to be the most effective chemical to get rid of veligers (planktonic larval form of quagga mussels) in the pipes. However, chlorine leaves a residue called trihalomethane, which is a carcinogen at higher concentrations. The purpose of this project is to test the effectiveness of an alternate chemical, chloramines (chlorine and ammonia), which leaves behind little to no residual trihalomethane. Upon experimentation with …

Evaluating Species Responses To Climate Change Using Ecological Niche Modeling And Genetic Data, Jeanette Perry

Undergraduate Research Opportunities Program (UROP)

The current and projected future warming trends together with degradation of habitats throughout much of the Great Basin and Columbian Plateau represent real threats to many species occupying these regions. If we can determine the impacts of past climatic changes on the distribution of species, we can obtain a better understanding of the future impacts of projected climatic trends on many species in these regions. My results with the Great Basin Pocket Mouse (Perognathus parvus) may be relevant to conservation ecologists and resource managers attempting to protect several Endangered Species Act candidates, such as the pygmy cottontail (Brachylagus idahoensis). I …

Effects Of Climate Change On The Viability Of The Devils Hole Pupfish, Matthew Heuton, Stanley D. Hillyard, Frank Van Breukelen

Undergraduate Research Opportunities Program (UROP)

Devils hole is an unusual, cavernous, aquifer-fed its ideal temperature range. spring in Death Valley National Park. It is the only home to a critically endangered species, Cyprinidon diabolis, a.k.a. The Devils Hole Pupfish. C. diabolis allows us a unique look at the impact of climate change on a species that has no gene flow and no possibility of escape from its current habitat. Climate change affects ectotherms (cold-blooded animals) tremendously since their body temperature is subject to changes in the temperature of their environment. In the case of C. diabolis, they are subject to high temperatures year round, around …

Oral Presentation: Plant Genes And Drought Tolerance, Norris Lam

Festival of Communities: UG Symposium (Posters)

Research has shown that a gene from C3 xerophyte Larrea tridentata (creosote bush), LtWRKY21, is involved in pathways governing creosote bush’s high tolerance to environmental stress. By understanding the way in which creosote bush adapts to drought, crop plants can be engineered to be more drought tolerant during times of imminent global climate change. To study the underlying mechanisms of creosote bush drought response, the LtWRKY21 gene was mobilized into the model organism Arabidopsis thaliana. Chlorophyll degradation, cellular electrolyte leakage, and water content in leaves will serve as indicators of drought tolerance in LtWRKY21-transgenic A. thaliana after treatment in chemically …

Has Recent Climate Change Caused A Genetic Bottleneck In A Sierra Nevada Population Of The Bushy-Tailed Woodrat?, Mitchell Gritts, Angela D. Hornsby, Majorie D. Matocq

Festival of Communities: UG Symposium (Posters)

Many montane species respond to climate change by shifting their range upslope as temperatures at lower elevations increase. An elevation range shift causes a range contraction that may result in a population bottleneck. Joseph Grinnell surveyed the fauna along the Yosemite transect from 1914 to 1920. In 2003 Craig Moritz and his colleagues began to resurvey the Yosemite transect to assess the faunal change during a century of climate change. The bushy-tailed woodrat suffered severe range contraction and population bottleneck between the two surveys. I will use evolutionary models to determine if the population has suffered a genetic bottleneck.

Stationary Phase Mutagenesis In Bacillus Subtilisis Independent Of Genome Replication, Mark Upchurch, Holly Martin, Eduardo Robleto

Festival of Communities: UG Symposium (Posters)

Stationary phase mutagenesis is defined as cellular mechanisms that produce genetic diversity in cells experiencing conditions of stress. These processes are associated with many biological phenomena, including those that produce the formation of cancers in animal cells and other degenerative diseases. Also, these mechanisms are associated with the accumulation of beneficial mutations in bacteria, but follow stochastic processes and are controlled by genetic factors. The current models explaining the generation of stress-induced mutations are predicated on the formation of DNA replication intermediates that are formed during the repair of damaged DNA or during DNA replication and transcription encounters. Here we …

Does Thermal Stress Cause Females Of The Plant Bryum Argenteum To Manipulate The Sex Ratios Of Their Offspring?, Elisha Rhodes, Lloyd Stark

Festival of Communities: UG Symposium (Posters)

About half of all moss taxa exhibit female-biased sex ratios, and bryophyte male rarity remains largely unexplained. One possible explanation is differential stress tolerance of spores due to maternal sporophyte manipulation during stress. To test this hypothesis, sporophytes of the species Bryum argenteum were subjected to thermal stress and observed for growth abnormalities and sporophyte abortions. Data display a correlation between increased temperature and increased sporophyte abortions as well as increased time to complete meiosis, possibly indicating decreased fitness. Continued global warming may create more stressful environments for sporophytes resulting in the elimination of males from local populations.

Similarity Of Climate Change Data For Antarctica And Nevada, Corbin Benally, Shahram Latifi, Karletta Chief

Undergraduate Research Opportunities Program (UROP)

The correlation between temperature and carbon dioxide concentration in the past one hundred years is studied. Separate graphs containing data from Vostok, Antarctica and the Mojave desert/mountain west (Nevada region) are presented. Using data obtained from these graphs, an attempt is made to explain the results and investigate the similarity of these results for Antarctica and Nevada. The importance of this study lies in the fact that if data show the same trend in the two regions, many findings for climate change in Antarctica may readily be validated and employed for Nevada.

The Endosymbiotic Relationship Of Leguminosae (Fabaceae) And Rhizobium, Sabrina Mcgee, Dolores Maceda, Maria Bustos, Maryknoll Palisoc, Rachel Dahl

Undergraduate Research Opportunities Program (UROP)

This project will look at The Endosymbiotic Relationship of Leguminosae (Fabaceae) and Rizobioum. Endoysmbiosis Theory is the concept that mitochondria and chloroplasts are the result of years of evolution initiated by the endocytosis, (the process by which cells absorb material through their cell membrane) of bacteria and algae which, instead of becoming digested, became symbiotic.The mitochondria of eukaryotes evolved from aerobic bacteria, probably rickettsias, living within their host cell and the chloroplasts from cyanobacteria, also known as blue-green algae. Endoysmbiosis is a type of symbiosis in which one organism lives inside the body of another and both function as a …

Assessing Invasive Plant Species As Threats In Mojave Desert Parks, Sara L. Mcpherson, Donovan J. Craig, Scott R. Abella

Graduate Research Symposium (GCUA) (2010 - 2017)

The invasion and persistence of exotic plant species threatens the natural features that national parks are designed to protect. For example, park managers have witnessed an increase in the frequency, extent and intensity of fires along with a reduction in native species richness and diversity. Many park managers are familiar with a suite of highly invasive plants, but lack a comprehensive and systematic way of prioritizing invasive plant species based on potential threats to the parks’ resources. We have entered into a collaborative project with the National Park Service’s Inventory and Monitoring program to develop an invasive plant early detection …

29 Years Of Vegetation Community Change Across Environmental Gradients In A Mojave Desert Mountain Range, Christopher L. Roberts, James S. Holland, Scott R. Abella

Graduate Research Symposium (GCUA) (2010 - 2017)

There is a great deal of uncertainty as to how biological communities respond to changes in land use and climate change, a situation particularly relevant in protected areas such as national parks that were designated to conserve specific biological features. Utilizing extant vegetation data sets with repeatable methodology can provide opportunities for insight into previous vegetation change and provide base line data for long-term monitoring projects useful for modeling vegetation community trajectories. We have relocated and resurveyed 106 sites from a vegetation community study initiated in 1979 in the Newberry Mountains, southern Nevada, within Lake Mead National Recreation Area managed …

Water Source Partitioning For Shrubland Transpiration Using Innovative Field Methods, Dale A. Devitt, Michael Young, Matthew S. Lachniet, Jeremy Koonce, Amanda Wagner, Brian M. Bird, J. Healey

2010 Annual Nevada NSF EPSCoR Climate Change Conference

37 PowerPoint slides Convener: Franco Biondi, UNR & Michael Young, DRI Session 4: Ecological Change and Water Resources Abstract: -Climate change models predict a decline in precipitation over the next few decades throughout much of the southwest. -Such change has the potential to shift water uptake dynamics of phreatophytes -If groundwater pumping also occurs, the impact of climate change could be exacerbated. -A better understanding of the forces that drive the coupling and decoupling of phreatophytes to groundwater is needed.

Design And Status Of The Elevationl Transect And Monitoring Systems For Nevada’S Nsf Epscor Climate Change Research Program, Brian M. Bird, Scotty Strachan, David B. Simeral, Richard L. Jasoni

2010 Annual Nevada NSF EPSCoR Climate Change Conference

15 PowerPoint slides Convener: Franco Biondi, UNR & Michael Young, DRI Session 4: Ecological Change and Water Resources Abstract: -Current status of Transects -Sheep Range Transect (5 sites) fully permitted through Fish and Wildlife Service. -Site access agreement acquired from the Long Now Foundation for Snake Range (sites 1, 2 and 3). -Snake Range site 4 access granted through the Nevada Land Conservatory. -Waiting for site permit approval on sites 8,9 and 6,7 from BLM and GBNP respectively. -Tower installed at North Las Vegas UWCC for testing of sensors and communications. -Installation of towers will begin winter 2010 at lower …

Biotic Responses To Climate Change In The Great Basin And Mojave Desert: Vegetation, David Charlet, Patrick Leary

2010 Annual Nevada NSF EPSCoR Climate Change Conference

84 PowerPoint slides Convener: Franco Biondi, UNR & Michael Young, DRI Session 4: Ecological Change and Water Resources Abstract: -The Problem -To detect changes in vegetation as it responds to changes in climate, we must first know where the vegetation is now -GAP (Geographic Approach to Protection of Biodiversity) map --National project to map land cover throughout the US. --Nevada map (Edwards et al. 1996) -Southwest ReGAP map --Land cover map for southwestern US (Prior-Magee et al. 2007) to correct problems identified in GAP map

Geovisualization Of Ecological Data For Park Policy Support, Scott R. Abella, Haroon Stephen, Ross Guida

2010 Annual Nevada NSF EPSCoR Climate Change Conference

12 PowerPoint slides Convener: William Smith, UNLV Session 3: Policy, Decision Making, and Outreach Abstract: -Literature shows upward elevation shifts of biological species as a result of climate change -Effects of climate change expected to accelerate in coming decades -Concern both about species migrating out of parks and the potential inability of species to quickly adapt to the changing conditions within National Parks and other federal land boundaries

Research Poster: Physiological Responses Of Two Invasive Annual Grasses, Cheatgrass And Red Brome, In The Great Basin, L. Hernandez, R. Nowak, L. Salto

2010 Annual Nevada NSF EPSCoR Climate Change Conference

Research poster

Research Poster: Vegetation Change In The Newberry Mountains Of Southern Nevada, Ross Guida, William J. Smith Jr., Scott R. Abella

2010 Annual Nevada NSF EPSCoR Climate Change Conference

Research poster

Research Poster: Climate Change Impacts To Groundwater, Springs Hydrology And Aquatic Communities Amargosa Desert And Death Valley National Park, Nevada And California, Terry Fisk, Greg Pohll, Don Sada, Mark Stone

2010 Annual Nevada NSF EPSCoR Climate Change Conference

Research poster

Research Poster: Climate Change Science For Northern Nevada Educators, Melissa Slayden, Jacque Ewing-Taylor, Kelly Cannon

2010 Annual Nevada NSF EPSCoR Climate Change Conference

Research poster

Research Poster: Quaternary Biogeography Of Neotoma Cinerea: Linking Genetic Patterns With Environmental Change, Angela D. Hornsby, Majorie D. Matocq

2010 Annual Nevada NSF EPSCoR Climate Change Conference

Research poster

Research Poster: Clark County School District Involvement In The Nsf Epscor Program, Education Component, Aubrey M. Shirk, Larry Rudd, Paul Buck

2010 Annual Nevada NSF EPSCoR Climate Change Conference

Research poster

Research Poster: Tree Population Dynamics At Ash Meadows National Wildlife Refuge: Influences Of Environmental Stress And Disturbance, Sarah L. Karam, Peter J. Weisberg, Stephanie O. Sunderman

2010 Annual Nevada NSF EPSCoR Climate Change Conference

Research poster

Research Poster: Water Source Partitioning For Shrubland Transpiration Using Innovative Field Methods, Amanda Wagner, Dale A. Devitt, Michael Young, Matthew S. Lachniet, Jeremy Koonce, Brian M. Bird

2010 Annual Nevada NSF EPSCoR Climate Change Conference

Research poster

Research Poster: Biotic Responses To Climate Change In The Mojave Desert: Floristics, Patrick Leary, David Charlet

2010 Annual Nevada NSF EPSCoR Climate Change Conference

Research poster

Climate Change Education For Nevada, David M. Hassenzahl, Michael Collopy, John W. Farley, Paul Buck, Jacque Ewing-Taylor, Shama Perveen

2010 Annual Nevada NSF EPSCoR Climate Change Conference

42 PowerPoint slides Session 1: Education Convener: David Hassenzahl, UNLV Abstract: -Five Year Strategic Plan -Goal 6 - Create a scholarly environment to promote research skills and intellectual development for Nevada educators and students (K-12, undergraduate undergraduate, and graduate) -Primary Strategy - Develop educational infrastructure to train students at all levels and provide public outreach in climate change issues