Life Sciences Commons^™

Examining Population Structure Of Cismontane And Desert Populations Of Zebra-Tailed Lizards (Callisaurus Draconoides) Using Mitochondrial And Nuclear Intron Dna., Lauren Nicole Morrison

Electronic Theses, Projects, and Dissertations

Callisaurus draconoides, also known as the Zebra-Tailed lizard, belongs to the family Phrynosomatidae family (Pianka, et al. 1972). C. draconoides is a widespread desert lizard found western North America. In California, this species can be found in the Mojave and Colorado Deserts. There are currently several populations that reside in the San Bernardino basin on the cismontane side of the Transverse and Peninsular ranges. These mountain ranges have the potential to have isolated the cismontane populations from their typical desert ranges. In addition, geological passes have the potential to serve as migration corridor between the Deserts and cismontane regions. The …

Attack Of The Clones: Elucidating The Role Of Clonality In The Invasion Success Of Carpobrotus Edulis, Eduardo Luis Cruz

Electronic Theses, Projects, and Dissertations

Transcriptomics is a modern technique in genomics that utilizes RNA sequences to get a snapshot of genetic expression. This is a powerful tool in non-model species lacking a reference genome. Thus, the application of comparative transcriptomics has the potential to help us elucidate the evolutionary mechanisms that facilitate species invasion. Carpobrotus edulis is a prolific and widespread invasive succulent plant belonging to the Aizoaceae family. A native to South Africa, this species has become a dominant invader of many Mediterranean coastal areas. In this study, we leveraged the use of RNAseq to investigate evolutionary changes among invasive populations. RNA-seq data …

Potential Fitness Trade-Offs Of Elevated Esterase Activity Associated With Insecticide Resistance In The Mosquito Culex Quinquefasciatus, Timothy Schulte

Electronic Theses, Projects, and Dissertations

One of the current major issues in the control of vector-borne disease is resistance to insecticides. Resistance to one or more insecticides has appeared in over 580 different arthropod species. Widespread resistance has led to the field of Insecticide Resistance Management (IRM). The goal of IRM is to develop insecticide-use strategies that prevent or slow down the evolution of resistance. Computer models have shown that more effective IRM strategies rely on the assumption that resistance carries a fitness cost in the absence of insecticide use.

In the mosquito Culex quinquefasciatus resistance to certain organophosphate insecticides is caused by an increased …

Investigation Into The Evolution Of Heterogeneity Within Secondary Replicons And Their Maintenance In Genus Variovorax, Christopher Ne Ville

Electronic Theses, Projects, and Dissertations

Approximately 10% of all bacterial genomes sequenced thus far contain a secondary replicon. This considerable genetic reservoir contains many potentially mobilizable elements, allowing for the formation of many unique secondary replicons. This property of bacterial populations vastly increases the genomic diversity available to species that effectively take up and maintain these replicons. Members of the genus Variovorax have extensive heterogeneity in genome architecture, including sequenced isolates containing plasmids, megaplasmids, and chromids. Using available Illumina data on the NCBI database, we have completed these assemblies using 3^rd generation sequencing methods on 17 members of this genus. We have sequenced, assembled, …

Population Structure Of A Federally Endangered Plant (Astragalus Jaegerianus Munz, Fabaceae) With Limited Range Using Microsatellites, Sueann Neal

Electronic Theses, Projects, and Dissertations

Studies on population genetics examine the relationship and effects of population structure, migration, gene flow and demographic history, and are therefore important in the conservation of endangered species. Astragalus jaegerianus, a critically federally endangered species found in a geographically restricted range is investigated to determine population structure and genetic variation. Previous research on A. jaegerianus focused on DNA sequence data for cpDNA and nrDNA showed no variation. Further research on A. jaegerianus utilizing AFLP’s on the whole genome indicated substantial gene diversity and population structure consistent with geographically widespread species. AFLP research is a cost-effective process to identify levels …

Isolation Of Caldatribacterium (Op9) And Investigation Of Its Potential Interactions With A Novel, Co-Cultivated Thermodesulfobacterium Species, Toshio Alvarado

Electronic Theses, Projects, and Dissertations

Atribacteria (OP9), candidate phylum with no representatives in pure culture, is found in various anaerobic environments worldwide. “Caldatribacterium”, a lineage within Atribacteria that is predicted to be a strictly anaerobic sugar fermenter based on cultivation-independent genomic analyses, is currently being maintained in lab enrichment cultures with fucose as its sole growth substrate. Metagenomics and 16S rRNA gene tag sequencing indicated that the fucose culture was a co-culture of “Caldatribacterium” and an uncultivated member of the genus Thermodesulfobacterium. Due to failed attempts to isolate “Caldatribacterium” by dilution-to-extinction and plating, it was hypothesized that “Caldatribacterium” is dependent in some way on the …

Using Single-Cell Sorting, Fish And 13c-Labeling To Cultivate And Assess Carbon Substrate Utilization Of ‘Aigarchaeota’ And Other Novel Thermophiles, Damon Kurtis Mosier

Electronic Theses, Projects, and Dissertations

‘Aigarchaeota’, a deeply branching lineage in the domain Archaea with no cultivated representatives, includes both thermophilic and hyperthermophilic microorganisms that reside in terrestrial and marine geothermal environments. The ‘Aigarchaeota’ consists of at least nine proposed genus-level groups that have been confirmed via 16S rRNA sequencing, with ‘Aigarchaeota’ Group 1 (AigG1) being the focus of this study. Based on cultivation-independent genomic data available from several AigG1 members in Great Boiling Spring (GBS), NV, and Yellowstone National Park, 22 different types of growth media were designed and tested for their ability to support growth of AigG1. One of these cultures, G1-10, was …

Geographic Population Structure And Taxonomic Identity Of Rhinichthys Osculus, The Santa Ana Speckled Dace, As Elucidated By Nuclear Dna Intron Sequencing, Liane Raynette Greaver

Electronic Theses, Projects, and Dissertations

Rhinichthys osculus (Cyprinidae), the speckled dace, is the most widely distributed freshwater fish in the western United States. The southern California populations of R. osculus are identified as the Santa Ana speckled dace (SASD), though the SASD has not yet been formally recognized as a distinct taxon. Current mtDNA analysis performed in the Metcalf Lab has shown a reciprocally monophyletic relationship among three California regions; southern, central coast, and Owens Valley. Similarly, microsatellite genotyping has shown significant levels of geographic population structure. The purpose of this study was to provide nuclear DNA sequence data to determine the taxonomic status of …

Redescription And Phylogenetic Analysis Of The Materials Assigned To The Taxon "Captorhinikos" Chozaensis, Jason Paul Jung

Electronic Theses, Projects, and Dissertations

“Captorhinikos” chozaensis is a multiple-tooth-rowed captorhinid reptile from the Lower Permian Clear Fork Group, undivided formation. Upon re-examination of the materials associated with the species from both the Chicago Field Museum of Natural History, and the Smithsonian United States National Museum, I reaffirm their affinity and collective identity as a valid taxon. “Captorhinikos” chozaensis does not, however, belong with either of the two members of its genus, C. valensis or “C.” parvus, instead occupying its own branch on the phylogenetic tree of the Captorhinidae. This conclusion is based in strong results from a combined phylogenetic parsimony analysis combined with …

The Santa Ana Speckled Dace (Rhinichthys Osculus): Phylogeography And Molecular Evolution Of The Mitochondrial Dna Control Region, James Jay Vanmeter

Electronic Theses, Projects, and Dissertations

The purpose of this genetic study of the Santa Ana Speckled Dace Rhinichthys osculus was three-fold. The first goal was to characterize the molecular structure of the mtDNA control region of R. osculus. An 1143 base-pair region of the mitochondrial DNA genome, which included the complete control region was sequenced for all individuals. Analysis of the sequence data revealed that the molecular structure of the speckled dace control region was similar to the molecular structure described for other vertebrate taxa. The speckled dace control region contains three major domains, which vary in base frequency as well as in the frequency …

Molecular Evolution And Phylogeography Of Mitochondrial Dna Cytochrome B Gene In Southern California Santa Ana Speckled Dace (Rhinichthys Osculus), Pia Marie Vanmeter

Electronic Theses, Projects, and Dissertations

In this study, I sequenced the mitochondrial cytochrome b gene to elucidate the extent and pattern of genetic variations among and within populations of Rhinichthys osculus (Santa Ana Speckled Dace) found in the different watersheds in Southern California, Central California Coast and Eastern California Desert. I described and analyzed the structural characteristics and pattern of base sequence substitutions in the cytochrome b gene to understand the molecular evolution of the gene. The Phylogenetic analyses showed that the Southern California Santa Ana Speckled dace is a distinct population from the Central California Coast dace population and Eastern California Desert dace population, …

Microsatellite Analysis Of Population Structure In The Santa Ana Speckled Dace (Rhinicthys Osculus), Stacey A. Nerkowski

Electronic Theses, Projects, and Dissertations

Rhinichthys osculus, the Speckled Dace, is one of the most ubiquitous fish in western North America. Within the Southern California region, the local taxon is known as the Santa Ana Speckled Dace. The purpose of this study was to characterize and identify polymorphic microsatellite markers for R. osculus in which twenty-three were identified through Illumina pair-end sequencing. Seven of these loci were then used to examine the patterns of genetic variation and population structure that occurred within and among the watersheds in the Southern California. The study also examined the regional relationships among Southern California, Central California and Owen’s …

A Review Of The Removal Of Arundo Donax From A Riparian Area Within San Timoteo Canyon, Catherine E. Howe

Electronic Theses, Projects, and Dissertations

A site within San Timoteo Canyon was revisited, 13-14 years after treatment, to look at long-term effects of Arundo donax removal. The data obtained were used to determine whether A. donax had re-invaded, other non-native species had established the area, or if native species were able to grow in place of the removed A. donax. The previous removals included a combination of grinding large patches of A. donax and then foliar spraying, foliar spraying of uncut plants, and direct spraying of hand cut stems, depending upon the location and size of the plant. The effects of the A. donax …

An Evolutionary Psychology Perspective On Responsibility Attributions For Infidelity And Relationship Dissolution, Kindra Lynn Edmonson

Theses Digitization Project

This study investigated responsibility attributions for a partner's emotional infidelity and for a partner's sexual infidelity, and the likelyhood that the victim or partner would end the relationship. This study found a significant relationship between responsibility attributions for a romantic partner's unfaithfulness and the likelihood the relationship would end: the stronger the attributions of personal responsibility the more likely the relationship would dissolve.

Alpine Ecology Of A C4 Grass, Muhlenbergia Richardsonis In The White Mountains Of California, Archie Thomas Meyer

Theses Digitization Project

The purpose of this thesis was to understand how this unusual C₄ alpine grass, Muhlenbergia Richardsonis, persists in the alpine zone along the western slope of California's White Mountains.

Comparative Osteohistology Of Hyperelongate Neural Spines In Basal Synapsids (Vertebrata, Amniota): Growth And Mechanical Considerations, Adam Keith Huttenlocker

Theses Digitization Project

Basal synapsids comprise one of the best represented groups of early tetrapods and demonstrate a classic example of a macroevolutionary transition. The basal synapsid fossil record is of significant interest to paleontolists because it presents an excellent opportunity for studying the evolution of vertebrate morphology. In the present study, neural spine osteohistology is further examined across a broad range of basal synapsid genera with hyperlongate neural spines. Due to the limited availability of specimens and the comparative approach required for destructive analysis, the present study is divided into two major comparative investigations: analysis of hyperlongate neural spine in (1) Spenacodontidae …

Effects Of Natural History On Osmoregulatory Behaviors In Two Stream-Dwelling Frogs (Pseudacris Cadaverina And P. Regilla), Heidy Lorena Contreras

Theses Digitization Project

Differences in osmoregulatory behaviors were studied in two stream-dwelling tree frogs (Pseudacris cadaverina and P. regilla) with different natural histories. This study supports the idea that the natural history of a species has a strong effect on behavior associated with osmoregulation.

A Geographically Constrained Molecular Phylogeny Of Panamanian Aechmea Species (Bromeliaceae, Subfamily Bromelioideae), Keri Renee Maher

Theses Digitization Project

This study lends strong support to the idea that members of Bromeliaceae have undergone a recent adaptive radiation, and therefore show that, at least in part, diversity in the tropics is due to a fast speciation rate and that the tropics can be a "cradle" for new diversification and exploitation of varying ecological niches through the diversification of ecophysiological traits within a lineage.

Population Genetics And Phylogeography Of The Pygmy Nuthatch In Southern California, Thomas Alan Benson

Theses Digitization Project

Uses mitochondrial DNA sequence data to examine the degree of genetic differentiation among sky island populations of two subspecies of pygmy nuthatch (Sitta pygmaea melanotis and Sitta pygmaea leuconucha) occurring in Southern California. Assesses the hypothesis that sky islands are genetically isolated and that migration (gene flow) is restricted among them. Eleven sampling locations throughout Southern California were selected based on representation of subspecies, availability of specimens, and feasibility of collection. Results indicate that pygmy nuthatch populations fragmented in the disjunct mountain ranges of Southern California exhibit low but significant levels of genetic differentiation.

Analysis Of Molecular Variation In The Federally Endangered Astragalus Jaegerianus (Fabaceae, Papilionoideae): A Species With A Restricted Geographic Range, George Floyd Walker

Theses Digitization Project

The purpose of this study is to investigate the level and distribution of genetic variation in Astragalus jaegerianus by using molecular markers. The objectives of the study are: to estimate levels of genetic variation within and among populations of Astragalus jaegerianus; to test the hypothesis that levels and patterns of genetic variation in species of restricted ranges and few individuals is low and partitioned at the population level; and to discern whether, or how well, genetic partitioning of Lane Mountain milk vetch correlates with its geographic partitioning in the field.

The Postcranial Skeleton Of The Family Limnoscelidae And Its Taxonomic Implications For Understanding Basal Amniotes, Natalia Kazimiera Wideman

Theses Digitization Project

The Diadectomorpha is a central taxon in understanding the origin and early evolution of amniotes. It is considered a sister taxon to Amniota and is so similar to amniotes that some researchers have placed it within the Amniota itself. This group is composed of three families: the Limnoscelidae, the Tseajaiidae, and the Diadectidae. Being the most basal member of this group, the family Limnoscelidae is especially important in these studies.

Thermal And Reproductive Biology Of The Lizards Sceloporus Occidentalis And Sceloporus Graciosus Along An Environmental And Geographical Gradient, Scott Landsborough Parker

Theses Digitization Project

The thermal environment exerts a strong influence on many aspectsof squamate life history. Environmental temperatures constrain daily activity time, fecundity, growth rate, and also influences hatchling phenotype. Cold nest temperatures can slow the development of eggs deposited in the soil, cause developmental abnormalities or result in mortality of embryos.

Ecological Correlates Of Protein Polymorphisms In A Desert And Non-Desert Population Of Belonia Saturata (Odonata: Anisoptera), Melissa Jean Hamilton

Theses Digitization Project

Two populations of the Big Red Skimmer Dragonfly, Belonia saturata (Odonata: Amisoptera), were studied to determine the thoracic temperatures maintained during flight, the presence of polymorphic enzyme systems, and to determine the differences in activity of glycerol-3-phosphate dehydrogenase between a desert and non-desert population.

Temporal Variation Of Esterase Activity Associated With Three Amplified Esterase Genes In A Field Population Of Culex Quinquefasciatus, Janine Marie Milligan

Theses Digitization Project

No abstract provided.

Heat Shock Proteins In Mojave Desert Dragonflies, Carol Juanita Promisel

Theses Digitization Project

Temperature plays a crucial role in the constant interaction between organisms and the environment. It affects development and rates of physiological functions as well as survival of organisms.

Vegetative Habitat Analysis Of Proposed Mine Sites In The Mojave Desert: The First Step Towards Revegetation Of Disturbed Desert Communities, Jim Van Brunt

Theses Digitization Project

No abstract provided.

Investigation Of Lower Colorado River Valley Desert Soil Mineral And Nutrient Content In Relation To Plant Proximity And Identity, Jane N. Hildreth

Theses Digitization Project

No abstract provided.

A Floristic And Vegetational Analysis Of The Mill Creek Drainage Area Of The San Bernardino Mountains, California, Carol Elaine O'Casey

Theses Digitization Project

No abstract provided.

A Study Of Changes In Algal Population Density Diversity And Distribution And Changes In Physical And Chemical Characteristics Of Lake Elsinore, Robert H. Nyman

Theses Digitization Project

No abstract provided.

Band Pattern In Helix Aspersa: Variation, Selection And Microgeographic Distribution, John. Elliott

Theses Digitization Project

No abstract provided.