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Computational Analysis Of Chromophore Tripeptides Followingfusion Of Enhanced Green Fluorescent Protein And Cell-Penetrating Peptides, Silvia Tri Widyaningtyas, Ekawati Betty Pratiwi, Budiman Bela
Computational Analysis Of Chromophore Tripeptides Followingfusion Of Enhanced Green Fluorescent Protein And Cell-Penetrating Peptides, Silvia Tri Widyaningtyas, Ekawati Betty Pratiwi, Budiman Bela
Makara Journal of Science
Cell-penetrating peptides (CPPs) are small peptides that can transfer other materials into a cellular compartment. In this research, we studied the effect of fusion of new CPPs to the N-terminal of enhanced Green Fluorescent Protein eGFP on the ability of the latter to fluoresce. Results showed that the recombinant protein CPPs-eGFP could be successfully expressed in Escherichia coli. In contrast to E. coli expressing wild-type eGFP, which could fluoresce under ultraviolet (UV) or visible light, E. coli expressing CPPs-eGFP lost their ability to fluoresce. PyMol, a molecular visualization system, revealed that fusion of the new CPPs to the N-terminal …