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Lack Of Processing Of The Expressed Orf1 Gene Product Of Hepatitis E Virus, Suganthi Suppiah, Yumei Zhou, Teryl K. Frey
Lack Of Processing Of The Expressed Orf1 Gene Product Of Hepatitis E Virus, Suganthi Suppiah, Yumei Zhou, Teryl K. Frey
Biology Faculty Publications
Background: Proteolytic processing is a common mechanism among plus strand RNA viruses and the replicases of all plus strand RNA viruses of animals thus far characterized undergo such processing. The replicase proteins of hepatitis E virus (HEV) are encoded by ORF1. A previous report published by our group [1] provided data that processing potentially occurred when ORF1 (Burma strain; genotype 1) was expressed using a vaccinia virus-based expression system.
Findings: To further test for processing and to rule out artifacts associated with the expression system, ORF1 was re-expressed using a plasmid-based expression vector with the result that the previous processing …