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Full-Text Articles in Life Sciences
Construction And Characterization Of A Chimeric Virus (Biv/Hiv-1) Carrying The Bovine Immunodeficiency Virus Gag-Pol Gene: Research Letters, Guomin Chen, Shuhui Wang, Kun Xiong, Jinzhong Wang, Tao Ye, Wenping Dong, Qi Wang, Qimin Chen, Yunqi Geng, Charles Wood, Yi Zeng
Construction And Characterization Of A Chimeric Virus (Biv/Hiv-1) Carrying The Bovine Immunodeficiency Virus Gag-Pol Gene: Research Letters, Guomin Chen, Shuhui Wang, Kun Xiong, Jinzhong Wang, Tao Ye, Wenping Dong, Qi Wang, Qimin Chen, Yunqi Geng, Charles Wood, Yi Zeng
Nebraska Center for Virology: Faculty Publications
HIV-1HXB2 5′LTR region, most of BIVR29 gag-pol segment and HIV-1HXB2 pol IN-3′LTR region were respectively amplified. A chimeric clone, designated as pHBIV3753, was constructed by cloning three fragments sequentially into pUC18. MT4 cells were transfected with pHBIV3753. The replication and expressions of the chimeric virus (HBIV3753) were monitored by RT activity and IFA. The results firstly demonstrated that it is possible to generate a new type of the BIV/HIV-1 chimeric virus containing BIV gag-pol gene.