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Actin Regulation And Furrow Dynamics During Early Drosophila Embryogenesis, Yi Xie
Actin Regulation And Furrow Dynamics During Early Drosophila Embryogenesis, Yi Xie
Electronic Theses and Dissertations
Drosophila embryogenesis starts with a single nucleus undergo 13 rounds of nuclear divisions called syncytial cycles. Staring at cycle 10 when nuclei migrate to the surface of the embryo, massive and dynamic cortical actin structures and cleavage furrow formations occur. How actin regulators coordinate into an organized network directing three-dimension actin structures in the developing organisms is an unsolved question. Here, I present an in-depth characterization of actin cap dynamics: the actin caps go through expansion, stabilization, elongation and fragmentation phases in each cycle. Arp2/3 is the major contributor to actin cap formation. The functions of 7 different actin and …
Rab8 Directs Tubulation And Furrow Ingression During Epithelial Formation In Drosophila Melanogaster, Lauren Mackenzie Mavor
Rab8 Directs Tubulation And Furrow Ingression During Epithelial Formation In Drosophila Melanogaster, Lauren Mackenzie Mavor
Electronic Theses and Dissertations
One of the most fundamental changes in cell morphology is the formation of a plasma membrane furrow. The Drosophila embryo undergoes several cycles of rapid furrow ingression during early development, which culminates in the formation of an epithelial sheet. Previous studies have demonstrated the requirement for intracellular trafficking pathways in furrow ingression; however, the pathways that link compartmental behaviors with cortical furrow ingression events have remained unclear. This research shows that Rab8, a small GTPase associated with late exocytic trafficking events, demonstrates striking dynamic behaviors in vivo; transitioning from punctate structures to a stable association with the cortex during …