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A High-Content Screen Identifies Hsp90 As A Component Of Axon Injury Signaling, Scott Karney-Grobe

Arts & Sciences Electronic Theses and Dissertations

Axons are unique compartments of neurons that extend tremendous distances throughout the body. Neurodegenerative or traumatic damage to axons destroys neuronal function and thus necessitates axonal repair. Mammalian peripheral neurons can regrow their axons following injury, providing a robust system to study the molecular mechanisms of axon regeneration. Injury induces a large transcriptional program that drives regeneration. While many regeneration-associated genes are known, the mechanisms by which injury activates them are less well understood. Identification of new injury signals has been limited by in vivo approaches. To address this challenge, I developed and performed a loss-of-function pharmacological screen in cultured …