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Developmental Biology

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2008

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Full-Text Articles in Life Sciences

Gfp Tagging Of Sieve Element Occlusion (Seo) Proteins Results In Green Fluorescent Forisomes, Hélène Pélissier, Winfried Peters, Ray Collier, Aart Van Bel, Michael Knoblauch Sep 2008

Gfp Tagging Of Sieve Element Occlusion (Seo) Proteins Results In Green Fluorescent Forisomes, Hélène Pélissier, Winfried Peters, Ray Collier, Aart Van Bel, Michael Knoblauch

Winfried S. Peters

Forisomes are Ca2+-driven, ATP-independent contractile protein bodies that reversibly occlude sieve elements in faboid legumes. They apparently consist of at least three proteins; potential candidates have been described previously as ‘FOR’ proteins. We isolated three genes from Medicago truncatula that correspond to the putative forisome proteins and expressed their green fluorescent protein (GFP) fusion products in Vicia faba and Glycine max using the composite plant methodology. In both species, expression of any of the constructs resulted in homogenously fluorescent forisomes that formed sieve tube plugs upon stimulation; no GFP fluorescence occurred elsewhere. Isolated fluorescent forisomes reacted to Ca2+ and chelators …

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Gene Expression Reveals Unique Skeletal Patterning In The Limb Of The Direct-Developing Frog, Eleutherodactylus Coqui, Ryan Kerney, James Hanken Dec 2007

Gene Expression Reveals Unique Skeletal Patterning In The Limb Of The Direct-Developing Frog, Eleutherodactylus Coqui, Ryan Kerney, James Hanken

Ryan Kerney

The growing field of skeletal developmental biology provides new molecular markers for the cellular pre- cursors of cartilage and bone. These markers enable resolution of early features of skeletal development that are otherwise undetectable through conventional staining tech- niques. This study investigates mRNA distributions of skeletal regulators runx2 and sox9 along with the cartilage-dominant collagen 2a1 (col2a1) in embryonic limbs of the direct- developing anuran, Eleutherodactylus coqui. To date, distri- butions of these genes in the limb have only been examined in studies of the two primary amniote models, mouse and chicken. In E. coqui, expression of transcription factors runx2 …

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