Life Sciences Commons^™

Igf-1 Reduce Protein O-Glcnacylation Via Ampk Activation In H9c2 Cardiomyoblast Cultured In High Glucose Media, Andres Medina, Lizbeth Trevino, Alejandra Salinas, Yadira Chaires, Erick Rodriguez, Genaro Antonio Ramirez-Correa

Research Colloquium

Background: Diabetes is a disease characterized by the increase in the serum glucose concentration. Therefore, this glucose excess shunts into the hexosamine biosynthetic pathway producing UDP-GlcNAc, which can be transfer to serine residues and modify the activity of different proteins, including contractile proteins in cardiomyocytes, decreasing their function. Here we show the effect of IGF-1 treatment on this posttranslational modification.

Methods: H9c2 cell line were cultured in DMEM media, 10% FBS, at 80% of confluence. Cells were incubated in high-glucose (30 mM) in the presence or absence of 10 µmol/L of IGF-1 (HG and HG+IGF-1). As control groups, we used …

Endothelial Cell Nrf2-Ko Attenuates Endothelial Function And Skeletal Muscle Antioxidant Capacity, Elizabeth J. Pekas, Cody P. Anderson, Michael F. Allen, Kollyn B. Jallen, Dong Gun Jin, Andres Benitez-Albiter, Christian D. Sanchez Tato, Lie Gao, Neha Dhyani, Tara L. Rudebush, Irving H. Zucker, Song-Young Park

UNO Student Research and Creative Activity Fair

INTRODUCTION: Endothelial cells line the inner surface of blood vessels and play a major role in modulating blood flow and gas exchange. Endothelial dysfunction is thought to be a contributor to cardiovascular disease development, and it is well-accepted that excessive reactive oxygen species (harmful molecules) likely contribute to endothelial dysfunction. The transcription factor nuclear factor erythroid 2-related factor 2 (Nrf2) is considered the master regulator of cellular protection in response to elevated reactive oxygen species. Therefore, Nrf2 may be a potential therapeutic target to protect against endothelial dysfunction. However, the roles of endothelial cell-specific Nrf2 on endothelial function are not …

Impaired Hydrogen Sulfide Protein Expression In Patients With Peripheral Artery Disease, Dong Gun Jin, Kollyn B. Jallen, Liz Pekas, Song-Young Park

UNO Student Research and Creative Activity Fair

INTRODUCTION: Hydrogen sulfide (H₂S) is a gaseous signaling molecule that serves various roles in the vasculature, such as upregulating angiogenesis, vascular smooth muscle relaxation, protecting endothelial function, and regulating redox balance. Despite H₂S’s positive impacts on vascular homeostasis, it is important to note that its actions depend on its concentrations. At high concentrations, H₂S has been reported to increase oxidative stress damage, such as oxidation of cysteine residues and lipid peroxidation. This may indicate that H₂S may act as a ‘double-edged sword’ in the field of vascular physiology. Peripheral artery disease (PAD) …

Prevention Of Doxorubicin-Induced Cardiotoxicity By A Coumestan Analogue, Psoralidin, Mujtaba H. Shah, Myia Aiges, Kota V. Ramana

Annual Research Symposium

Natural phenolic compound Psoralidin limits cardiotoxicity associated with Doxorubicin