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- Aerobic exercise (1)
- Afterhyperpolarization (1)
- Aging (1)
- Alzheimer's disease (1)
- Calcium Dysregulation (1)
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- Cocaine degradation (1)
- Cocaine esterase (1)
- Cocaine use disorder (1)
- E196-301 stability (1)
- Electrophysiology (1)
- Energy compensation (1)
- Hippocampus (1)
- Hunger hormones (1)
- RBP 8000 stability (1)
- Red blood cells as drug delivery system (1)
- Rootlets (1)
- Stereocilia actin core repair (1)
- Temporary noise-induced hearing loss (1)
- Temporary threshold shifts (1)
- Weight loss (1)
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Articles 1 - 4 of 4
Full-Text Articles in Life Sciences
Efficient Cocaine Degradation By Cocaine Esterase-Loaded Red Blood Cells, Luigia Rossi, Francesca Pierigè, Marco Agostini, Noemi Bigini, Veronica Termopoli, Yingting Cai, Fang Zheng, Chang-Guo Zhan, Donald W. Landry, Mauro Magnani
Efficient Cocaine Degradation By Cocaine Esterase-Loaded Red Blood Cells, Luigia Rossi, Francesca Pierigè, Marco Agostini, Noemi Bigini, Veronica Termopoli, Yingting Cai, Fang Zheng, Chang-Guo Zhan, Donald W. Landry, Mauro Magnani
Molecular Modeling and Biopharmaceutical Center Faculty Publications
Recombinant bacterial cocaine esterase (CocE) represents a potential protein therapeutic for cocaine use disorder treatment. Unfortunately, the native enzyme was highly unstable and the corresponding mutagenized derivatives, RBP-8000 and E196-301, although improving in vitro thermo-stability and in vivo half-life, were a partial solution to the problem. For cocaine use disorder treatment, an efficient cocaine-metabolizing enzyme with a longer residence time in circulation would be needed. We investigated in vitro the possibility of developing red blood cells (RBCs) loaded with RBP-8000 and E196-301 as a biocompatible system to metabolize cocaine for a longer period of time. RBP 8000 stability within human …
Divergence In Neuronal Calcium Dysregulation In Brain Aging And Animal Models Of Ad, Adam Ghoweri
Divergence In Neuronal Calcium Dysregulation In Brain Aging And Animal Models Of Ad, Adam Ghoweri
Theses and Dissertations--Pharmacology and Nutritional Sciences
Neuronal calcium dysregulation first garnered attention during the mid-1980’s as a key factor in brain aging, which led to the formulation of the Ca2+ hypothesis of brain aging and dementia. Indeed, many Ca2+-dependent cellular processes that change with age, including an increase in the afterhyperpolarization, a decrease in long-term potentiation, an increased susceptibility to long-term depression, and a reduction in short-term synaptic plasticity, have been identified. It was later determined that increased intracellular Ca2+ with age was due to increased Ca2+ channel density, elevated release from intracellular Ca2+ stores, and decreased Ca2+ buffering …
Remodeling In The Actin Core Of The Auditory Hair Cell Stereocilia As A Novel Component Of Temporary Noise-Induced Hearing Loss, Jonathan Michael Grossheim
Remodeling In The Actin Core Of The Auditory Hair Cell Stereocilia As A Novel Component Of Temporary Noise-Induced Hearing Loss, Jonathan Michael Grossheim
Theses and Dissertations--Physiology
The rigid, paracrystalline actin core of auditory hair cell stereocilia is extremely stable and after initial formation must persist for the life of the cell to preserve hearing in mammals. In healthy hair cells, turnover of actin molecules occurs only in a small region near the tips of stereocilia, while the actin filaments of the shaft are stable. For decades damage to the actin core of stereocilia from acoustic trauma has only been attributed to cases of permanent noise-induced hearing loss. Here, we show that repairable actin core damage occurs in temporary noise-induced hearing loss from moderate acoustic trauma.
We …
Appetite-Regulating Hormones In Energy Compensation With Exercise, Jack Moreland
Appetite-Regulating Hormones In Energy Compensation With Exercise, Jack Moreland
Theses and Dissertations--Nutrition and Food Systems
Background: The appetite-regulating hormones may influence compensatory increases in energy intake with exercise, although this causal relationship has been difficult to prove in a longitudinal trial.
Methods: 37 participants (29 female) aged 18 to 40 years performed aerobic exercise 6 days (6d), 2 days (2d), or 0 days per week for 12-weeks. Concentrations of ghrelin, leptin, glucagon-like peptide 1 (GLP-1), and insulin were assessed before (fasting, minute 0) and after a standardized meal at minute 15, 30, 45, 60, 90, 120, 150, and 180. Linear mixed-effects models were used to model the relationships between time point (12 weeks vs. baseline) …