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Full-Text Articles in Life Sciences
Deciphering The Role Of Mitochondrial Physiology And Thermal Acclimation In Shaping Whole Organismal Performance Of An Invasive Forest Pest, Essa Alrashdi
Masters Theses
Environmental factors such as temperature are substantial determinants of the spongy moth, L. dispar, distribution, reproduction, and growth. Accumulating energy reserves at the larval stage is particularly important to L. dispar, since the larvae metamorphose into a fully-grown, non-feeding adult. As non-feeding adults, the energy balance of the pupae must be adequate, to ensure enough energy reserves for adult dispersal, egg maturation and overall persistence of the species in the region. At this stage, environmental temperature also determines daily metabolic demands, and the overall cost of sustaining ecologically relevant activities. Various hypotheses describing a mismatched growth and metabolic …
Seasonal Temperature Changes In Three Midwestern Wastewater Streams And The Effects On Fish Assemblages And Physiology, Ryan William Sparks
Seasonal Temperature Changes In Three Midwestern Wastewater Streams And The Effects On Fish Assemblages And Physiology, Ryan William Sparks
Masters Theses
Midwestern United States undergo extreme temperature and precipitation fluctuations throughout the year, which leads to changing habitats within Illinois river systems. Wastewater treatment facilities have long been used to treat sewage and reduce the organic products in sewage before releasing it into aquatic environments. Wastewater treatment facilities must maintain optimal conditions, especially temperature, for reproduction and growth of facilitated bacteria that break down organic materials in wastewater. The objectives for this study were to determine the thermal effects of wastewater treatment facilities on receiving waters; and secondly, to investigate the influence of these wastewater facilities on fish abundance and assemblages. …
The Role Of Creg1 As A Master Regulator Of Liver Function, Abdulrahman Siran Aldaghmi
The Role Of Creg1 As A Master Regulator Of Liver Function, Abdulrahman Siran Aldaghmi
Masters Theses
The liver is known as the chemical factory of the body because it performs a wide range of biochemical functions required for life. Since the liver has such an important role in regulation of normal physiological processes, liver diseases cause a high rate of morbidity and mortality. Therefore, understanding the mechanisms of liver development will shed light on the causes of liver disease. In this study, a cell line model that utilizes rat hepatoma cells (Fg14) and hepatoma variant cells (H11) was used to identify master regulators of liver gene expression. Whole genome expression studies identified the gene CREG1 (Cellular …
Metabolic And Morphologic Shifts In Neuro2a Cells Cultured In Galactose Medium, Leah Welker
Metabolic And Morphologic Shifts In Neuro2a Cells Cultured In Galactose Medium, Leah Welker
Masters Theses
It has been observed that highly-proliferating cells, such as cancer cells, rely mainly on glycolysis for ATP production, regardless of presence of oxygen. This effect, however, can be reversed by changing the main energy substrate in the medium from glucose to galactose. The oxidation of galactose in glycolysis yields less net ATP, presumably forcing the cell into OXPHOS. This has been established in many cell lines, including HeLA, HepG2, and skeletal muscle cells. As of yet, this has not been reproduced in neuronal cells. Using Neuro2a, a murine neuroblastoma cell line, this study exposes neuronal cells to galactose medium, and …
D-Galactose Decreases Mitoneet Levels In Liver Cancer Cells: Impact On Cellular Bioenergetics, Sudip Paudel
D-Galactose Decreases Mitoneet Levels In Liver Cancer Cells: Impact On Cellular Bioenergetics, Sudip Paudel
Masters Theses
Galactose is a simple sugar that at supraphysiological concentrations accelerates aging and age-related complications, which lead to impaired mitochondrial functions. MitoNEET is a small mitochondrial membrane protein with a molecular mass of 12.2 kDa that functions in diabetes, iron metabolism, regulation of oxidative phosphorylation (OXPHOS) and reactive oxygen species (ROS) homeostasis. MitoNEET knockdown increases cellular respiration rates and ROS levels similar to galactose treatment. Pioglitazone, an antidiabetic drug, binds to mitoNEET and ameliorates galactose toxicity. Cellular mitoNEET levels, exposure of cells to galactose medium, and pioglitazone treatment directly influence cellular respiration. To elucidate the role of mitoNEET in galactose induced …
The Effect Of Ergosterol On The Desaturation Of 14c-Labeled Oleic Acid In Tetrahymena, Maynard E. Neville Jr.
The Effect Of Ergosterol On The Desaturation Of 14c-Labeled Oleic Acid In Tetrahymena, Maynard E. Neville Jr.
Masters Theses
The ciliated protozoan Tetrahymena pyriformis-Strain W desaturated 14C-labeled fatty acid substrate in both sterol supplemented (ergosterol) and nonsupplemented cultures. Cis-octadecenoic acid (14C-9-18:1), when added to the medium during the log phase of growth, was incorporated into the cells and also desaturated at the Δ6 and Δ12 positions. The 14C labeled substrate and products, linoleate (9,12-18:2) or linolenate (6,9,12-18:3) were recovered and separated by silver-nitrate Unisil column chromatography. Initially, recovery of 14C compounds varied widely when expressed as the percent of total counts per minute recovered from column chromotography, and a variety of …