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Full-Text Articles in Life Sciences
Neurodegenerative Modeling: Tau Protein, Degradative Pathways, And Gene Expression Profiling Of Human Ipsc-Derived Neural Precursors And Differentiated 3-D Neural Sphere Versus 2-D Monolayer Cultures, Kyle H. Anthoney
Cal Poly Humboldt theses and projects
Human induced pluripotent stem cells offer a model for human brain development and disease by differentiation into brain organoids; however, current neural culture systems lack the microenvironment, neuronal circuits and connectivity, vascular circulation, and immune system that exist in vivo. After differentiation and development of neuronal and non-neuronal cell types within two formats of cell cultures, we can visualize and recapitulate in vivo protein accumulation, gene expression, and degradative processes such as autophagy. Using RNA extraction, purification methods and reverse transcription I compared traditional monolayer cultures and novel 3-D neural sphere cultures via gene expression analysis. This analysis indicated …
Effects Of Gap Junction Blockage On Regenerating Body Segments Of Lumbriculus Variegatus, Monica K. Richmond
Effects Of Gap Junction Blockage On Regenerating Body Segments Of Lumbriculus Variegatus, Monica K. Richmond
Cal Poly Humboldt theses and projects
Lumbriculus variegatus is a freshwater annelid that is well known for its regenerative capacity. There are many different factors that affect regeneration in animals. Cell-to-cell communication is a key component of regeneration. Gap junctions are made up of proteins that create a channel that connects the cytoplasm of two cells. Many molecules and ions pass through the gap junctions that can affect regeneration. The purpose of this study was to determine if blocking gap junctions would have an impact on regenerating body segments of L. variegatus and what effects it would produce. L. variegatus was exposed to a known gap …