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Tattletales And T-Bow Update 20140602mon, George Mcnamara
Tattletales And T-Bow Update 20140602mon, George Mcnamara
George McNamara
Tattletales and T-Bow Update 20140602Mon
http://works.bepress.com/gmcnamara/42
Please see also http://works.bepress.com/gmcnamara/26
Tattletales: multiplex fluorescent protein biosensors by spatial localization with TALE-FPs, Cas9-FPs, ZF-FPs, LacI-FPs, TetR-FPs, etc.
T-Bow: Rainbow T-cells and Tumor cells (and ES cells, iPS cells, other cells and organisms). You can think of this as "Brainbow meets TALENs/Cas9/ZFNs/other DNA sequence specific binding proteins".
If not familiar with Brainbow, see
http://en.wikipedia.org/wiki/Brainbow
If not familiar with TALENs, Cas9, etc, see
http://www.addgene.org/genome_engineering/
Big idea: localizing fluorescent proteins - and/or Nano-Lanterns (Take Nagai) - to tandem repeat arrays - is a great way to improve signal to noise ratio compared to the usual …
Pubspectra Tattletales, George Mcnamara
Pubspectra Tattletales, George Mcnamara
George McNamara
Tattletales for Multiplex Fluorescent Reporters in Single Cells for Metabolomics
George McNamara
As of April 2013: L.J.N. Cooper & D.A. Lee Cellular Immunotherapy Lab, University of Texas M.D. Anderson Cancer Center, Houston, TX
Email: gtmcnamara@mdanderson.org, geomcnamara@earthlink.net
Tattletales is my concept for spatial multiplexing many fluorescent protein (FP) biosensors in the same live cell. For example, there are excellent FP biosensors to Ca++ ions, pH, glucose, ribose, glutamine, glutamate, ATP, redox, ROS, pyruvate, cAMP, cGMP, IP3, PI(3,4,5)P3, cell cycle indicators (Fucci2), PKA, PKC, photsphatases, caspase(s) [1, 2]. However, these are typically used one biosensor per experiment, due in part to flooding …