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Articles 1 - 7 of 7
Full-Text Articles in Life Sciences
Monoclonal Antibodies To Novel Myeloid Antigens Reveal Human Neutrophil Heterogeneity., Edward D. Ball, Robert F. Graziano, Li Shen, Michael W. Fanger
Monoclonal Antibodies To Novel Myeloid Antigens Reveal Human Neutrophil Heterogeneity., Edward D. Ball, Robert F. Graziano, Li Shen, Michael W. Fanger
Dartmouth Scholarship
Three cytotoxic murine monoclonal antibodies that recognize myeloid-specific antigens have been produced by immunization with normal human neutrophils or myeloblasts from a patient with acute myelomonocytic leukemia. Two of these, PMN 6 and PMN 29, are specific for neutrophils; the third monoclonal antibody, AML-2-23, is reactive with the majority of normal monocytes as well as a subpopulation of mature neutrophils. Although neutrophils from all individuals tested expressed these antigens, cytofluorographic analysis revealed that the percentage of cells bearing the PMN 6 and AML-2-23 antigens varied among individuals. Significant additional heterogeneity in the density of each antigen among antigen-bearing cells was …
In Vivo Study Of The Effects Of Danazol On Cytoplasmic Receptors In The Female Rat, Deborah L. Spencer
In Vivo Study Of The Effects Of Danazol On Cytoplasmic Receptors In The Female Rat, Deborah L. Spencer
Morehead State Theses and Dissertations
A thesis presented to the faculty of the School of Sciences and Mathematics at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science in Biology by Deborah L. Spencer on August 26, 1982.
A New Column Perfusion Technique Using Isolated Rat Adrenal Cells To Study Acth And Danazol, Diane H. Johnson
A New Column Perfusion Technique Using Isolated Rat Adrenal Cells To Study Acth And Danazol, Diane H. Johnson
Morehead State Theses and Dissertations
A thesis presented to the faculty of the School of Sciences and Mathematics at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science in Biology by Diane H. Johnson on August 15, 1982.
Factors Affecting Thyrotropin Secretion In Superfused Rat Anterior Pituitary Cells, Frederick Scott Bartlow
Factors Affecting Thyrotropin Secretion In Superfused Rat Anterior Pituitary Cells, Frederick Scott Bartlow
Dissertations and Theses
The use of static in vitro pituitary cultures has been indispensible for examining the regulation of anterior pituitary hormone secretion. While the static cultures have shown the effects of various chemical stimulators of hormone secretion, the dynamics of such secretion has not been assessed before in vitro. The in vitro superfusion method, which partially stimulates in vivo physiologic conditions, allows for the observation of the dynamics of pituitary hormone secretion.
Partial Reconstruction Of The Microvillus Core Bundle: Characterization Of Villin As A Ca(++)-Dependent, Actin-Bundling/Depolymerizing Protein, Paul T. Matsudaira, David Burgess
Partial Reconstruction Of The Microvillus Core Bundle: Characterization Of Villin As A Ca(++)-Dependent, Actin-Bundling/Depolymerizing Protein, Paul T. Matsudaira, David Burgess
Dartmouth Scholarship
The brush border, isolated from chicken intestine epithelial cells, contains the 95,000 relative molecular mass (M(r)) polypeptide, villin. This report describes the purification and characterization of villin as a Ca(++)-dependent, actin bundling/depolymerizing protein. Then 100,000 g supernatant from a Ca(++) extract of isolated brush borders is composed of three polypeptides of 95,000 (villin), 68,000 (fimbrin), and 42,000 M(r) (actin). Villin, following purification from this extract by differential ammonium sulfate precipitation and ion-exchange chromatography, was mixed with skeletal muscle F-actin. Electron microscopy of negatively stained preparations of these villin-actin mixtures showed that filament bundles were present. This viscosity, sedimentability, and ultrastructural …
Dark Repair Of Lethal Damage Induced In A Hybrid Mammalian Tissue Culture Cell Line By Ultraviolet Light, Robin Haetten, Margaret Mcguinness, H. Gaston Griggs
Dark Repair Of Lethal Damage Induced In A Hybrid Mammalian Tissue Culture Cell Line By Ultraviolet Light, Robin Haetten, Margaret Mcguinness, H. Gaston Griggs
Journal of the Arkansas Academy of Science
No abstract provided.
Electron Microscopic Demonstration Of Neural Connections Using Horseradish Peroxidase: A Comparison Of The Tetramethylbenzidine Procedure With Seven Other Histochemical Methods, Keith A. Carson, M-Marsel Mesulam
Electron Microscopic Demonstration Of Neural Connections Using Horseradish Peroxidase: A Comparison Of The Tetramethylbenzidine Procedure With Seven Other Histochemical Methods, Keith A. Carson, M-Marsel Mesulam
Biological Sciences Faculty Publications
Eight methods for the electron microscopic demonstration of horseradish peroxidase (HRP) labeling have been compared in adjacent series of vibratome sections of mouse lumbar spinal cord. The tracer, a HRP-wheat germ agglutinin (WGA) conjugate, was injected into the gastrocnemius muscle complex. Following retrograde axonal transport to the lumbar motor neurons and transganglionic anterograde transport of the tracer to the dorsal horn, the HRP activity was demonstrated in eight series of adjacent sections of lumbar spinal cord using eight methods. These included procedures using tetramethylbenzidine (TMB), benzidine dihydrochloride (BDHC), o-tolidine, paraphenylenediamine-pyrocatechol (PPD-PC), and 4 methods using 3,3'-diaminobenzidine (DAB). All eight methods …