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Pcdp1 Is A Central Apparatus Protein That Binds Ca2+-Calmodulin And Regulates Ciliary Motility, Christen G. Dipetrillo, Elizabeth F. Smith
Pcdp1 Is A Central Apparatus Protein That Binds Ca2+-Calmodulin And Regulates Ciliary Motility, Christen G. Dipetrillo, Elizabeth F. Smith
Dartmouth Scholarship
For all motile eukaryotic cilia and flagella, beating is regulated by changes in intraciliary calcium concentration. Although the mechanism for calcium regulation is not understood, numerous studies have shown that calmodulin (CaM) is a key axonemal calcium sensor. Using anti-CaM antibodies and Chlamydomonas reinhardtii axonemal extracts, we precipitated a complex that includes four polypeptides and that specifically interacts with CaM in high [Ca2+]. One of the complex members, FAP221, is an orthologue of mammalian Pcdp1 (primary ciliary dyskinesia protein 1). Both FAP221 and mammalian Pcdp1 specifically bind CaM in high [Ca2+]. Reduced expression of Pcdp1 complex …
Neuropilin 1 Directly Interacts With Fer Kinase To Mediate Semaphorin3a-Induced Death Of Cortical Neurons, Susan X. Jiang, Shawn N. Whitehead, Amy Aylsworth, Bogdan Zurakowski, Kenneth Chan, Jianjun Li, Sheng T. Hou
Neuropilin 1 Directly Interacts With Fer Kinase To Mediate Semaphorin3a-Induced Death Of Cortical Neurons, Susan X. Jiang, Shawn N. Whitehead, Amy Aylsworth, Bogdan Zurakowski, Kenneth Chan, Jianjun Li, Sheng T. Hou
Anatomy and Cell Biology Publications
Neuropilins (NRPs) are receptors for the major chemorepulsive axonal guidance cue semaphorins (Sema). The interaction of Sema3A/NRP1 during development leads to the collapse of growth cones. Here we show that Sema3A also induces death of cultured cortical neurons through NRP1. A specific NRP1 inhibitory peptide ameliorated Sema3A-evoked cortical axonal retraction and neuronal death. Moreover, Sema3A was also involved in cerebral ischemia-induced neuronal death. Expression levels of Sema3A and NRP1, but not NRP2, were significantly increased early during brain reperfusion following transient focal cerebral ischemia. NRP1 inhibitory peptide delivered to the ischemic brain was potently neuroprotective and prevented the loss of …