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Early Arrest Mutant Ogre: A Defect In Cytokineis Caused By A Mutation In The Gene Racgap1, April Wicklund
Early Arrest Mutant Ogre: A Defect In Cytokineis Caused By A Mutation In The Gene Racgap1, April Wicklund
Masters Theses
The early arrest mutant ogre, isolated in the Tübingen screen, has a block in cytokinesis (Kane et al., 1996). Here I use genetic and molecular techniques to demonstrate that the ogre phenotype is caused by a lesion in the gene encoding Racgap1. I performed fine mapping studies using microsatellite markers to a 0.15 cM region containing the ogre locus. DNA sequence data revealed a nonsense mutation in the Racgap1 gene of ogre mutants, and in situ hybridization experiments showed a loss of racgap1 mRNA at later stages of development, likely by nonsense mediated decay. This evidence supports the abolishment …
Role Of The Mitotic Cyclin Clb2 In Mitotic Regulation, Dustin K. Stutts
Role Of The Mitotic Cyclin Clb2 In Mitotic Regulation, Dustin K. Stutts
Masters Theses
In the budding yeast Saccharomyces cerevisiae, the mitotic cell cycle is regulated by the cyclin-dependent kinase (CDK) Cdc28. Cdc28 is activated by binding to one of nine cyclins, which then directs Cdc28’s function and localization. Clb2 is the main mitotic cyclin, promoting entry into mitosis and progression from the metaphase to anaphase transition. In order for cells to exit mitosis, CDK activity must decrease; CDK activity is regulated through Clb2 degradation. Degradation of Clb2 is mediated by the Anaphase-Promoting Complex (APC), which is an E3 ubiquitin ligase that is regulated by the spindle assembly checkpoint. The APC has two activators: …