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Full-Text Articles in Life Sciences
The Role Of Rock Signaling On Salivary Gland Organoid Formation, Matthew Koslow
The Role Of Rock Signaling On Salivary Gland Organoid Formation, Matthew Koslow
Legacy Theses & Dissertations (2009 - 2024)
Salivary glands are crucial organs that are responsible for secreting saliva which is important for the breakdown of carbohydrates, providing lubrication of the mouth, and maintenance of oral health. Patients diagnosed with Sjögren's Syndrome or receiving treatment for head and neck cancer, can permanently lose salivary gland function, which is referred to as salivary hypofunction. As a result, these patients develop Xerostomia, or the feeling of dry mouth, and suffer from an overall decreased quality of life. To combat the negative effects of salivary hypofunction, stem cell therapy is an attractive option, as implantation of specific cell populations can rescue …
Chromatin-Signaling Axis Orchestrates The Formation Of Germline Stem Cell Differentiation Niche In Drosophila, Maitreyi Upadhyay
Chromatin-Signaling Axis Orchestrates The Formation Of Germline Stem Cell Differentiation Niche In Drosophila, Maitreyi Upadhyay
Legacy Theses & Dissertations (2009 - 2024)
Stem cells have the unique capability of self-renewing into stem cells and differentiating into several terminal cell types. Loss of either of these processes can lead to aging, progression towards degenerative diseases and cancers. Insight into how self-renewal and differentiation are regulated will have tremendous therapeutic impact. Drosophila is an excellent model system for stem cell study due to the availability of various mutants, markers and RNAi technology. In order to study stem cell biology, we use female Drosophila gonads, whose stem cell population – the germline stem cells (GSCs) gives rise to gametes.
A Breast Cancer Stem Cell Model Created From Mmtv-Pymt Mice Applicable To Human Breast Cancer, Denise Grant Lanza
A Breast Cancer Stem Cell Model Created From Mmtv-Pymt Mice Applicable To Human Breast Cancer, Denise Grant Lanza
Legacy Theses & Dissertations (2009 - 2024)
Cancer stem cells are the seeds of tumor growth, but there are limited cell-based methods that exist to study the properties of these cells. To create a model of breast cancer stem cells, we isolated tumors from MMTV-PyMT mice. Two out of the four different cell types isolated survived in culture, CD44+CD24- and CD24+CD49f+CD44low. We found that we could initiate tumors with as few as 10 cells injected subcutaneously in the hind leg or orthotopically in the cleared fat pad with CD24+ cells. However, we could not initiate tumors with injection of CD24- cells. We found a requirement for TICs …