Life Sciences Commons^™

Mcnamara 201412 Nih Scap Innocentive Challenge Solution - T-Bow Rainbow T-Cells And Tumor Cells Spatial Multiplexing Gene Expression Reporter System – Plus Supplement Plus Posters - 20151027 - Please Download "75" Instead, George Mcnamara

George McNamara

McNamara 201412 NIH SCAP InnoCentive Challenge Solution - T-Bow Rainbow T-cells and Tumor Cells Spatial Multiplexing Gene Expression Reporter System – plus supplement plus posters - 20151027.

///

Please download the current 20151027 (October 27, 2015) Tattletales and T-Bow update from

http://works.bepress.com/gmcnamara/75/

The bepress web site is not letting me replace the old pdf here at "65" with the additional 10 pages update.

///

The download is my/Cooper lab solution (submission) to the 2014 NIH Single Cell Analysis Program (SCAP) InnoCentive Challenge, "Follow That Cell". I submitted the Solution on 20141215Mon (with 20 minutes to spare). The Challenge web page …

Tattletales And T-Bow Update 20151027tue, George Mcnamara

George McNamara

20151027Tue this "75"

http://works.bepress.com/gmcnamara/75

is my update of "65" posting

See text at

http://works.bepress.com/gmcnamara/65/

for text summary. The PDf here in "75" supersedes "65".

The PDF here has 10 pages added to the end from the "65" version (pages 40-49 of PDF when including the bepress cover page)..

here is the text in my cover page (bepress may add its own cover):

20151027Tue: added 10 page e-poster at bottom explaining Binary Tattletales and T-Bow. That is, binary with respect to protein components. For one color (number of repeats, epitope tags, FPs are examples, here rounded to convenient numbers):

1. 100 …

Gpu Deconvolution Wow Result On 2048x2048x32 Plane Z-Series, George Mcnamara

George McNamara

GPU Deconvolution WOW result on 2048x2048x32 plane Z-series ... formerly bad academic code ("you get what you pay for") now impressive

Alternative title: "instant gratification quantitative deconvolution fluorescence microscopy".

http://works.bepress.com/gmcnamara/55/

Please see "74"

http://works.bepress.com/gmcnamara/74/

for 32-bit images from this project (bepress file size limitation prevented me from including them in this ZIP archive).

//

Summary: Deconvolution microscopy has historically been painfully slow. The early vendors were:

- Scanalytics (Carrington and Fay), commercialized to try to sell expensive, specialized array processors made by CSPI (the CSPI box likely had less computing power than a first gen smartphone).

- Applied Precision (Sedat …

32-Bit Deconvolution Files Accompanying 55 Page, George Mcnamara

George McNamara

ZIP file with 32-bit images accompanying

http://works.bepress.com/gmcnamara/55

Bepress apparently has an ~1 Gigabyte file upload limit so I have placed the 32-bit images of greatest interest here.

See Word document inside for details.

Mcnamara 2010 Metamatters Newletter Series - Rogers Pmn The Chase And Temporal Area Maps, George Mcnamara

George McNamara

My four 2010 MetaMorph MetaMatters newsletter articles on "The Chase" (video available for download here on my bepress site) and history / use of temporal area maps.

Light Microscopy Reflection Focusing Cells And Coverglass, George Mcnamara

George McNamara

Light Microscopy Reflection Focusing Cells and Coverglass 20150520Wed

Many light microscopes have the capability of using camera based brightfield, phase contrast, or fluorescence to find the most contrasty specimen focus. When I worked for UIC (1992-1997) we offered this with a combination of Ludl MAC2000 controller, Z-drive, video card thingy, and video camera (most customers at the time used analog video cameras).

Many automated light microscopes now have clever -- and pricey -- autofocus systems based on an NIR LED structured illumination reflecting light off the bottom of the coverglass.

I encourage a simpler approach - my hardware details are …