Life Sciences Commons^™

Contribution Of Advanced Fluorescence Nano Microscopy Towards Revealing Mitotic Chromosome Structure, S W. Botchway, Safana Farooq, A Sajid, I K. Robinson, Mohammed Yousuf

Centre for Regenerative Medicine & Stem Cell Research

The organization of chromatin into higher-order structures and its condensation process represent one of the key challenges in structural biology. This is important for elucidating several disease states. To address this long-standing problem, development of advanced imaging methods has played an essential role in providing understanding into mitotic chromosome structure and compaction. Amongst these are two fast evolving fluorescence imaging technologies, specifically fluorescence lifetime imaging (FLIM) and super-resolution microscopy (SRM). FLIM in particular has been lacking in the application of chromosome research while SRM has been successfully applied although not widely. Both these techniques are capable of providing fluorescence imaging …