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Full-Text Articles in Life Sciences
Budding Yeast Protein Extraction And Purification For The Study Of Function, Interactions, And Post-Translational Modifications, Eva P. Szymanski, Oliver Kerscher
Budding Yeast Protein Extraction And Purification For The Study Of Function, Interactions, And Post-Translational Modifications, Eva P. Szymanski, Oliver Kerscher
Arts & Sciences Articles
Homogenization by bead beating is a fast and efficient way to release DNA, RNA, proteins, and metabolites from budding yeast cells, which are notoriously hard to disrupt. Here we describe the use of a bead mill homogenizer for the extraction of proteins into buffers optimized to maintain the functions, interactions and post-translational modifications of proteins. Logarithmically growing cells expressing the protein of interest are grown in a liquid …
Developmental Plasticity In Response To Familiar And Unfamiliar Predators In The Mud Snail, Ilyanassa Obsoleta, Kelly Strickland Hoolihan
Developmental Plasticity In Response To Familiar And Unfamiliar Predators In The Mud Snail, Ilyanassa Obsoleta, Kelly Strickland Hoolihan
Dissertations, Theses, and Masters Projects
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