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- Drosophila (2)
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Articles 1 - 5 of 5
Full-Text Articles in Life Sciences
Regulation Of The Wnt/Β-Catenin Pathway By Steroid Hormones In Danio Rerio Ovarian Tissue, Macaulie Casey
Regulation Of The Wnt/Β-Catenin Pathway By Steroid Hormones In Danio Rerio Ovarian Tissue, Macaulie Casey
Summer Research
The Wnt/β-catenin signaling pathway regulates genes involved in proliferation (cell growth) and apoptosis (cell death). It has been implicated in ovarian cancer, where higher levels of β-catenin may be involved in the development of tumors. Steroid hormones play a significant role in female reproductive tissue, and studies have shown that estrogens and progestins may regulate the Wnt/β-catenin pathway. My past research suggested testosterone may also affect the pathway. Few studies have investigated how steroid hormone mimics, such as Bisphenol A (BPA), affect these regulatory patterns. This study investigated the affect of estrogen and BPA on the Wnt/β-catenin pathway in Danio …
Oncogene Characterization And Mapping, John Evans
Oncogene Characterization And Mapping, John Evans
Summer Research
New oncogenes can be uncovered using the UAS/GAL4 system in the model organism Drosophila Melanogaster. P-elements allow both UAS and GAL4 to insert into the genome of parental flies. When crossed, both UAS/GAL4 are transferred to the progeny and express both sequence and protein that result in cancerous phenotypes that are easily identifiable using light microscopy. Inverse PCR, sequence analysis and comparison to online databases, e.g. flybase.org, provides simple identification of the culprit gene.
Effect Of High Water Temperature On Apoptosis In Zebrafish (Danio Rerio) Ovarian Tissue, Sarah Mueller
Effect Of High Water Temperature On Apoptosis In Zebrafish (Danio Rerio) Ovarian Tissue, Sarah Mueller
Summer Research
Elevated water temperature has significant effects on the biology of fishes, which raises major concerns about the impacts of climate change on the health of fish populations. High water temperature has been shown to induce apoptosis, or programmed cell death, in various tissues of fish. However, the effects of high water temperature on apoptosis in the ovaries of fish has been little-studied, despite its implications for the overall reproductive health of fishes. This study addresses the effects of high water temperature on apoptosis in the ovarian tissue of zebrafish. qPCR was used to quantify the expression of genes related to …
The Role Of Mmp9 In Satellite Cell Activation After Increased Activity, Mike H.M.H. Pang, Jung A. Kim, Gary E. Mccall, Ryan S. Mehan, J-P Hyatt
The Role Of Mmp9 In Satellite Cell Activation After Increased Activity, Mike H.M.H. Pang, Jung A. Kim, Gary E. Mccall, Ryan S. Mehan, J-P Hyatt
Summer Research
The unique adaptive ability of skeletal muscle to meet functional demands is exemplified in its response to exercise. Though little is known about the molecular mechanisms that regulate this plasticity, the extracellular matrix (ECM) is believed to play a large role. The basal lamina is a specialized layer of ECM that lies in direct contact with the cell membrane of muscle fibers and facilitates environment-to-cell interactions. Matrix metalloproteinase-9 (MMP-9) is an enzyme in the basal lamina that regulates much of these adaptive processes. During exercise, the regenerative process of damaged tissue requires the activation of muscle-specific stem cells known as …
Effect Of Altered Cellular Redox Environment On Oncogenic Activity Of The Drosophila Prl Protein, Frances Welsh
Effect Of Altered Cellular Redox Environment On Oncogenic Activity Of The Drosophila Prl Protein, Frances Welsh
Summer Research
Aberrant expression of members of the phosphatase of regenerating liver (PRL) family has been implicated as a key factor in the progression of several forms of human cancers. However, despite a wide range of studies supporting the role of the enzyme PRL as an oncogene, it has also been identified as a growth suppressor when tested under different conditions. One proposed explanation for this change in function is that redox regulation controls the accessibility of the active site of PRLs, which is necessary for oncogenic output. In this study, cellular redox environment was altered in vivo using Drosophila melanogaster, …