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Surface Ruffles As Markers For Studies Of Cell Transformation By Rous Sarcoma Virus, Victor R. Ambros, Lan Bo Chen, John M. Buchanan
Surface Ruffles As Markers For Studies Of Cell Transformation By Rous Sarcoma Virus, Victor R. Ambros, Lan Bo Chen, John M. Buchanan
Victor R. Ambros
Confluent chick embryo fibroblasts infected with the Ts68 mutant of Rous sarcoma virus were examined by scanning electron microscopy at the permissive (36 degrees) and nonpermissive (41 degrees) temperatures for transformation. Infected cells shifted from 41 degrees to 36 degrees undergo a change in shape from elongated to rounded. This process is preceded by the appearance of surface ruffles on the cell. These surface ruffles are not observed on cells maintained at 41 degrees, appear as early as 0.5 hr after a shift to 36 degrees, and are common on cells maintained at 36 degrees. By 3.5 hr after the …