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Transcriptional Profiling Of Caulobacter Crescentus During Growth On Complex And Minimal Media, Craig Stephens, Alison K. Hottes, Maliwan Meewan, Desiree Yang, Naomi Arana, Pedro Romero, Harley H. Mcadams.
Transcriptional Profiling Of Caulobacter Crescentus During Growth On Complex And Minimal Media, Craig Stephens, Alison K. Hottes, Maliwan Meewan, Desiree Yang, Naomi Arana, Pedro Romero, Harley H. Mcadams.
Biology
Microarray analysis was used to examine gene expression in the freshwater oligotrophic bacterium Caulobacter crescentus during growth on three standard laboratory media, including peptone-yeast extract medium (PYE) and minimal salts medium with glucose or xylose as the carbon source. Nearly 400 genes (approximately 10% of the genome) varied significantly in expression between at least two of these media. The differentially expressed genes included many encoding transport systems, most notably diverse TonB-dependent outer membrane channels of unknown substrate specificity. Amino acid degradation pathways constituted the largest class of genes induced in PYE. In contrast, many of the genes upregulated in minimal …
Identification Of The Flii And Flij Components Of The Caulobacter Flagellar Type Iii Protein Secretion System, Craig Stephens, Chris Mohr, Charles Boyd, Janine Maddock, James Gober, Lucy Shapiro
Identification Of The Flii And Flij Components Of The Caulobacter Flagellar Type Iii Protein Secretion System, Craig Stephens, Chris Mohr, Charles Boyd, Janine Maddock, James Gober, Lucy Shapiro
Biology
Caulobacter crescentus is motile by virtue of a polar flagellum assembled during the predivisional stage of the cell cycle. Three mutant strains in which flagellar assembly was blocked at an early stage were isolated. The mutations in these strains mapped to an operon of two genes, fliI and fliJ, both of which are necessary for motility. fliI encodes a 50-kDa polypeptide whose sequence is closely related to that of the Salmonella typhimurium FliI protein, an ATPase thought to energize the export of flagellar subunits across the cytoplasmic membrane through a type III protein secretion system. fliJ encodes a 16-kDa hydrophilic …