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Evidence For The Requirement Of 14-3-3eta (Ywhah) In Meiotic Spindle Assembly During Mouse Oocyte Maturation, Santanu De, Douglas Kline
Evidence For The Requirement Of 14-3-3eta (Ywhah) In Meiotic Spindle Assembly During Mouse Oocyte Maturation, Santanu De, Douglas Kline
Biology Faculty Articles
Background
The 14-3-3 (YWHA) proteins are central mediators in various cellular signaling pathways regulating development and growth, including cell cycle regulation. We previously reported that all seven mammalian 14-3-3 isoforms are expressed in mouse oocytes and eggs and that, 14-3-3η (YWHAH) accumulates and co-localizes in the region of meiotic spindle in mouse eggs matured in vivo. Therefore, we investigated the role of 14-3-3η in spindle formation during mouse oocyte maturation.
Results
Examination of oocytes matured in vitro demonstrated that 14-3-3η accumulates in both meiosis I and II spindles. To explore if 14-3-3η interacts directly with α-tubulin in meiotic spindles, …
Expression Of 14-3-3 Protein Isoforms In Mouse Oocytes, Eggs And Ovarian Follicular Development, Santanu De, Jennifer Marcinkiewicz, Srinivasan Vijayaraghavan, Douglas Kline
Expression Of 14-3-3 Protein Isoforms In Mouse Oocytes, Eggs And Ovarian Follicular Development, Santanu De, Jennifer Marcinkiewicz, Srinivasan Vijayaraghavan, Douglas Kline
Biology Faculty Articles
Background
The 14-3-3 (YWHA) proteins are a highly conserved, ubiquitously expressed family of proteins. Seven mammalian isoforms of 14-3-3 are known (β, γ, ε, ζ, η, τ and, σ). These proteins associate with many intracellular proteins involved in a variety of cellular processes including regulation of the cell cycle, metabolism and protein trafficking. We are particularly interested in the role of 14-3-3 in meiosis in mammalian eggs and the role 14-3-3 proteins may play in ovarian function. Therefore, we examined the expression of 14-3-3 proteins in mouse oocyte and egg extracts by Western blotting after polyacrylamide gel electrophoresis, viewed fixed …