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Tunel Apoptotic Cell Detection In Stony Coral Tissue Loss Disease (Sctld): Evaluation Of Potential And Improvements, E. Murphy Mcdonald
Tunel Apoptotic Cell Detection In Stony Coral Tissue Loss Disease (Sctld): Evaluation Of Potential And Improvements, E. Murphy Mcdonald
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Stony coral tissue loss disease (SCTLD) is a highly lethal coral disease that has caused a dramatic loss of coral tissue along the Florida Reef Tract and throughout the Wider Caribbean. This study seeks to understand whether programmed cell death (apoptosis) is involved in the pathology of the highly virulent SCTLD tissue loss lesion. Tissues from diseased colonies of Pseudodiploria strigosa collected in 2018 and 2020 were stained using the terminal deoxyribonucleotidyltransferase (TdT) mediated dUTP-biotin nick end labeling (TUNEL) assay to visualize areas of programmed cell death. The archived tissue samples collected in 2018 exhibited a significantly higher degree of …