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Evidence For The Requirement Of 14-3-3eta (Ywhah) In Meiotic Spindle Assembly During Mouse Oocyte Maturation, Santanu De, Douglas Kline
Evidence For The Requirement Of 14-3-3eta (Ywhah) In Meiotic Spindle Assembly During Mouse Oocyte Maturation, Santanu De, Douglas Kline
Biology Faculty Articles
Background
The 14-3-3 (YWHA) proteins are central mediators in various cellular signaling pathways regulating development and growth, including cell cycle regulation. We previously reported that all seven mammalian 14-3-3 isoforms are expressed in mouse oocytes and eggs and that, 14-3-3η (YWHAH) accumulates and co-localizes in the region of meiotic spindle in mouse eggs matured in vivo. Therefore, we investigated the role of 14-3-3η in spindle formation during mouse oocyte maturation.
Results
Examination of oocytes matured in vitro demonstrated that 14-3-3η accumulates in both meiosis I and II spindles. To explore if 14-3-3η interacts directly with α-tubulin in meiotic spindles, …