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Silencing Of Nadph-Dependent Oxidoreductase Genes (Yqhd And Dkga) In Furfural-Resistant Ethanologenic Escherichia Coli, Elliot N. Miller, Laura R. Jarboe, L. P. Yomano, S. W. York, K. T. Shanmugam, L. O. Ingram
Silencing Of Nadph-Dependent Oxidoreductase Genes (Yqhd And Dkga) In Furfural-Resistant Ethanologenic Escherichia Coli, Elliot N. Miller, Laura R. Jarboe, L. P. Yomano, S. W. York, K. T. Shanmugam, L. O. Ingram
Laura R. Jarboe
Low concentrations of furfural are formed as a side product during the dilute acid hydrolysis of hemicellulose. Growth is inhibited by exposure to furfural but resumes after the complete reduction of furfural to the less toxic furfuryl alcohol. Growth-based selection was used to isolate a furfural-resistant mutant of ethanologenic Escherichia coli LY180, designated strain EMFR9. Based on mRNA expression levels in the parent and mutant in response to furfural challenge, genes encoding 12 oxidoreductases were found to vary by more than twofold (eight were higher in EMFR9; four were higher in the parent). All 12 genes were cloned. When expressed …