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Articles 1 - 6 of 6
Full-Text Articles in Life Sciences
Phenotypic And Genotypic Characterization Of Escherichia Coli Isolated From Untreated Surface Waters, Kai F. Hung, Steven L. Daniel, Kristopher J. Janezic, Blake Ferry, Eric W. Hendricks, Brian A. Janiga, Tiffany Johnson, Samantha Murphy, Morgan E. Roberts, Sarah M. Scott, Alexandra N. Theisen
Phenotypic And Genotypic Characterization Of Escherichia Coli Isolated From Untreated Surface Waters, Kai F. Hung, Steven L. Daniel, Kristopher J. Janezic, Blake Ferry, Eric W. Hendricks, Brian A. Janiga, Tiffany Johnson, Samantha Murphy, Morgan E. Roberts, Sarah M. Scott, Alexandra N. Theisen
Kai F. Hung
A common member of the intestinal microbiota in humans and animals is Escherichia coli. Based on the presence of virulence factors, E. coli can be potentially pathogenic. The focus of this study was to isolate E. coli from untreated surface waters (37 sites) in Illinois and Missouri and determine phenotypic and genotypic diversity among isolates. Water samples positive for fecal coliforms based on the Colisure® test were streaked directly onto Eosin Methylene Blue (EMB) agar (37°C) or transferred to EC broth (44.5°C). EC broth cultures producing gas were then streaked onto EMB agar. Forty-five isolates were identified as E. coli …
Using Comparative Genomics For Inquiry-Based Learning To Dissect Virulence Of Escherichia Coli O157:H7 And Yersinia Pestis, David J. Baumler, Lois M. Banta, Kai F. Hung, Jodi A. Schwarz, Eric L. Cabot, Jeremy D. Glasner, Nicole T. Perna
Using Comparative Genomics For Inquiry-Based Learning To Dissect Virulence Of Escherichia Coli O157:H7 And Yersinia Pestis, David J. Baumler, Lois M. Banta, Kai F. Hung, Jodi A. Schwarz, Eric L. Cabot, Jeremy D. Glasner, Nicole T. Perna
Kai F. Hung
Genomics and bioinformatics are topics of increasing interest in undergraduate biological science curricula. Many existing exercises focus on gene annotation and analysis of a single genome. In this paper, we present two educational modules designed to enable students to learn and apply fundamental concepts in comparative genomics using examples related to bacterial pathogenesis. Students first examine alignments of genomes of Escherichia coli O157:H7 strains isolated from three food-poisoning outbreaks using the multiple-genome alignment tool Mauve. Students investigate conservation of virulence factors using the Mauve viewer and by browsing annotations available at the A Systematic Annotation Package for Community Analysis of …
Molybdate Treatment And Sulfate Starvation Decrease Atp And Dna Levels In Ferroplasma Acidarmanus, Kai F. Hung
Molybdate Treatment And Sulfate Starvation Decrease Atp And Dna Levels In Ferroplasma Acidarmanus, Kai F. Hung
Kai F. Hung
Sulfate is a primary source of sulfur for most microbes and in some prokaryotes it is used an electron acceptor. The acidophile Ferroplasma acidarmanus (strain fer1) requires a minimum of 150 mM of a sulfate-containing salt for growth. Sulfate is assimilated by F. acidarmanus into proteins and reduced to form the volatile organic sulfur compounds methanethiol and dimethyldisulfide. In the absence of sulfate, cell death occurs by an unknown mechanism. In this study, cell viability and genomic DNA and ATP contents of F. acidarmanus were monitored in response to the absence of sulfate or the presence of sulfate and the …
Apoptosis Of Dedifferentiated Hepatoma Cells Is Independent Of Nf-Jb Activation In Response To Lps, M. Ryan Reidy, Janette Ellis, Erin A. Schmitz, David M. Kraus, Gary A. Bulla
Apoptosis Of Dedifferentiated Hepatoma Cells Is Independent Of Nf-Jb Activation In Response To Lps, M. Ryan Reidy, Janette Ellis, Erin A. Schmitz, David M. Kraus, Gary A. Bulla
Gary A. Bulla
Dedifferentiated hepatoma cells, in contrast to most other cell types including hepatoma cells, undergo apoptosis when treated with lipopolysaccharide (LPS) plus the protein synthesis inhibitor cycloheximide (CHx). We recently reported that the dedifferentiated hepatoma cells also exhibit a strong and prolonged NF-jB induction phenotype upon exposure to LPS, suggesting that NF-jB signaling may play a pro-survival role, as reported in several other cell systems. To test the role of NF-jB in preventing LPS-mediated apoptosis, we examined the dedifferentiated cell line M38. Results show that antioxidants strongly inhibited LPS + CHx-mediated cell death in the M38 cells, yet only modestly inhibited …
Dissociation Of The Hepatic Phenotype From Hnf4 And Hnf1x Expression, Gary A. Bulla, David M. Kraus
Dissociation Of The Hepatic Phenotype From Hnf4 And Hnf1x Expression, Gary A. Bulla, David M. Kraus
Gary A. Bulla
Dedifferentiated cells have served as tools to understand the molecular consequences of the loss of tissue-specific pathways. Here we report the characterization of one of these cell lines, M29, which lacks the liver-enriched HNF4-HNF1x pathway, in order to determine if this class of variant cell lines could provide additional information regarding requirements for tissue-type expression. We report that although the liver-specific x1-antitrypsin (a1AT) gene remains silent despite reactivation of the HNF4/HNF1x pathway in the M29 cells, the frequency of activation of an integrated x1AT-APRT transgene is increased 1000-fold in response to these transcription factors. The human x1AT locus (introduced via …
Expression Of Gp73, A Resident Golgi Membrane Protein, In Viral And Nonviral Liver Disease, Raleigh D. Kladney, Xiaoyen Cui, Gary A. Bulla, Elizabeth M. Brunt, Claus J. Fimmel
Expression Of Gp73, A Resident Golgi Membrane Protein, In Viral And Nonviral Liver Disease, Raleigh D. Kladney, Xiaoyen Cui, Gary A. Bulla, Elizabeth M. Brunt, Claus J. Fimmel
Gary A. Bulla
GP73 is a novel type II Golgi membrane protein of unknown function that is expressed in the hepatocytes of patients with adult giant-cell hepatitis (Gene 2000;249:53-65). Its expression pattern in human liver disease and the regulation of its expression in hepatocytes have not been systematically studied. The aims of the present study were to compare GP73 protein levels in viral and nonviral human liver disease and in normal livers, to identify its cellular sources, and to study the regulation of its expression in hepatoma cells in vitro. GP73 protein levels were quantitated in explant livers of patients with well-defined disease …