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Mycoplasma Contaminants Present In Exosome Preparations Induce Polyclonal B Cell Responses., Helen O'Neill, Ben Quah
Mycoplasma Contaminants Present In Exosome Preparations Induce Polyclonal B Cell Responses., Helen O'Neill, Ben Quah
Helen O'Neill
Exosome fractions of dendritic cells (DC) produced in long-term cultures (LTC) were found to contain Mycoplasma contaminants. In this study, Mycoplasma-infected, -uninfected, and -reinfected cultures of DC and control cell lines have been compared for their capacity to activate lymphocytes. Using differential centrifugation, size fractionation, and inhibition assays, it has been possible to map Mycoplasma to the exosome or vesicle fraction purified from culture supernatant (CSN). Mycoplasma fractions were shown to induce proliferation of B and not T cells. The B cell response was sensitive to mitomycin C and primaquine, both known antibiotics, but resistant to protease and DNase, suggesting …
The Immunogenicity Of Dendritic Cell-Derived Exosomes, Ben Quah, Helen O'Neill
The Immunogenicity Of Dendritic Cell-Derived Exosomes, Ben Quah, Helen O'Neill
Helen O'Neill
Exosome production represents an alternate endocytic pathway for secretion. Multivesicular endosomes (MVE) fuse with the plasma membrane expelling internal vesicles or exosomes from cells. Exosome production has been recently described for immune cells including B cells, dendritic cells (DC), mast cells, macrophages and T cells. Exosomes derived from some DC populations stimulate T lymphocyte proliferation in vitro and have potent capacity to generate anti-tumour immune responses in vivo. These reported studies have involved in vitro grown mature DC expanded from precursors with cytokines. However, immature DC produce higher numbers of exosomes than mature DC and this is thought to be …