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Full-Text Articles in Life Sciences
The Effects Of Decellularized Adipose Tissue Constructs On Mesenchymal Stromal/Stem Cell Phenotype And Pro-Angiogenic Secretory Function., Yehia Moharrem
The Effects Of Decellularized Adipose Tissue Constructs On Mesenchymal Stromal/Stem Cell Phenotype And Pro-Angiogenic Secretory Function., Yehia Moharrem
Electronic Thesis and Dissertation Repository
Due to limited treatment options for critical limb ischemia (CLI), cellular-based therapies have been investigated to induce blood vessel regeneration. Bone marrow-mesenchymal stromal/stem cells (BM-MSC) have shown pre-clinical success in animal models of CLI as they possess pro-angiogenic and immunomodulatory functions. However, clinical translation has been hindered by inadequate expansion and delivery strategies. This project aimed to characterize the phenotype and pro-angiogenic secretory function of BM-MSC on decellularized adipose tissue (DAT) bioscaffolds as expansion platforms. Compared to cells grown on tissue-culture plastic, DAT substrates supported BM-MSC growth, regenerative marker expression, and pro-angiogenic secretory function. Conditioned media generated by BM-MSC cultured …
Cytoplasmic Polyadenylation Element Binding Protein 2 Alternative Splicing Regulates Hif1Α During Chronic Hypoxia, Emily M. Mayo
Cytoplasmic Polyadenylation Element Binding Protein 2 Alternative Splicing Regulates Hif1Α During Chronic Hypoxia, Emily M. Mayo
USF Tampa Graduate Theses and Dissertations
Chronic pulmonary hypoxia commonly results in the sustained expression of HIF1 (hypoxia inducible factor 1), a heterodimeric transcription factor, that, if unrestrained, can result in dramatic vasculature remodeling, pulmonary arterial hypertension, and right-sided heart failure. Together, these pulmonary disorders cost approximately $100 billion annually to treat due to the limited therapeutic targets designed to inhibit HIF1 expression. In this study, we introduce a translational regulator of HIF1 expression, known as Cytosolic polyadenylation element binding proteins 2 (CPEB2). Our lab has previously demonstrated in cancer cells that alternatively spliced isoforms of CPEB2 regulate the translation of the HIF1 oxygen-dependent subunit, HIF1α, …