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Full-Text Articles in Life Sciences
Uvrd Participation In Nucleotide Excision Repair Is Required For The Recovery Of Dna Synthesis Following Uv-Induced Damage In Escherichia Coli, Kelley Nicole Newton, Charmain T. Courcelle, Justin Courcelle
Uvrd Participation In Nucleotide Excision Repair Is Required For The Recovery Of Dna Synthesis Following Uv-Induced Damage In Escherichia Coli, Kelley Nicole Newton, Charmain T. Courcelle, Justin Courcelle
Biology Faculty Publications and Presentations
UvrD is a DNA helicase that participates in nucleotide excision repair and several replication-associated processes, including methyl-directed mismatch repair and recombination. UvrD is capable of displacing oligonucleotides from synthetic forked DNA structures in vitro and is essential for viability in the absence of Rep, a helicase associated with processing replication forks. These observations have led others to propose that UvrD may promote fork regression and facilitate resetting of the replication fork following arrest. However, the molecular activity of UvrD at replication forks in vivo has not been directly examined. In this study, we characterized the role UvrD has in processing …
The Role Of Nucleotide Excision Repair In Restoring Replication Following Uv-Induced Damage In Escherichia Coli, Kelley Nicole Newton
The Role Of Nucleotide Excision Repair In Restoring Replication Following Uv-Induced Damage In Escherichia Coli, Kelley Nicole Newton
Dissertations and Theses
Following low levels of UV exposure, Escherichia coli cells deficient in nucleotide excision repair recover and synthesize DNA at near wild type levels, an observation that formed the basis of the post replication recombination repair model. In this study, we characterized the DNA synthesis that occurs following UV-irradiation in the absence of nucleotide excision repair and show that although this synthesis resumes at near wild type levels, it is coincident with a high degree of cell death. We confirm that the replication occurring under these conditions involves extensive levels of strand exchange. However, cells undergoing this form of replication accumulate …
Inefficient Replication Reduces Reca-Mediated Repair Of Uvdamaged Plasmids Introduced Into Competent Escherichia Coli, Harout Arthur Jeiranian, Charmain T. Courcelle, Justin Courcelle
Inefficient Replication Reduces Reca-Mediated Repair Of Uvdamaged Plasmids Introduced Into Competent Escherichia Coli, Harout Arthur Jeiranian, Charmain T. Courcelle, Justin Courcelle
Biology Faculty Publications and Presentations
Transformation of Escherichia coli with purified plasmids containing DNA damage is frequently used as a tool to characterize repair pathways that operate on chromosomes. In this study, we used an assay that allowed us to quantify plasmid survival and to compare how efficiently various repair pathways operate on plasmid DNA introduced into cells relative to their efficiency on chromosomal DNA. We observed distinct differences between the mechanisms operating on the transforming plasmid DNA and the chromosome. An average of one UV-induced lesion was sufficient to inactivate ColE1-based plasmids introduced into nucleotide excision repair mutants, suggesting an essential role for repair …