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Full-Text Articles in Life Sciences

Identification And Rna Binding Characterization Of Plant Virus Rna Silencing Suppressor Proteins, Jeff Vargason, Carissa J. Burch, Jesse W. Wilson Nov 2013

Identification And Rna Binding Characterization Of Plant Virus Rna Silencing Suppressor Proteins, Jeff Vargason, Carissa J. Burch, Jesse W. Wilson

Faculty Publications - Department of Biological & Molecular Science

Suppression is a common mechanism employed by viruses to evade the antiviral effects of the host’s RNA silencing pathway. The activity of suppression has commonly been localized to gene products in the virus, but the variety of mechanisms used in suppression by these viral proteins spans nearly the complete biochemical pathway of RNA silencing in the host. This review describes the agrofiltration assay and a slightly modified version of the agro-infiltration assay called co-infiltration, which are common methods used to observe RNA silencing and identify viral silencing suppressor proteins in plants, respectively. In addition, this review will provide an overview …


Quantitative Trait Loci Mapping Reveals Candidate Pathways Regulating Cell Cycle Duration In Plasmodium Falciparum, Heather B. Reilly Ayala, Mark A. Wacker, Geoffrey Siwo, Michael T. Ferdig Oct 2010

Quantitative Trait Loci Mapping Reveals Candidate Pathways Regulating Cell Cycle Duration In Plasmodium Falciparum, Heather B. Reilly Ayala, Mark A. Wacker, Geoffrey Siwo, Michael T. Ferdig

Faculty Publications - Department of Biological & Molecular Science

Background: Elevated parasite biomass in the human red blood cells can lead to increased malaria morbidity. The genes and mechanisms regulating growth and development of Plasmodium falciparum through its erythrocytic cycle are not well understood. We previously showed that strains HB3 and Dd2 diverge in their proliferation rates, and here use quantitative trait loci mapping in 34 progeny from a cross between these parent clones along with integrative bioinformatics to identify genetic loci and candidate genes that control divergences in cell cycle duration. Results: Genetic mapping of cell cycle duration revealed a four-locus genetic model, including a major genetic effect …