Life Sciences Commons^™

Distribution Of Micropredators In The Longleaf Pine Soil Microbiome, Faith Arends, Eithar Mirghani

Symposium of Student Scholars

Bacterial micropredators of the soil, like their larger-scale counterparts, play a key role in shaping their ecosystem and are thus of particular interest from an ecological standpoint. An important micropredator to study are Myxobacteria. Myxobacteria are gram-negative rod-shaped social bacteria that hunt in wolf packs to lyse their prey and facilitate their growth. Myxobacteria are a potential source of novel antibiotics, therefore, the isolation of new strains is of great interest. We investigated longleaf pine soil as a source of novel isolates. The Longleaf Pine Ecosystem is an endangered ecosystem that is of crucial importance due to its ability to …

The Role Of Long Non-Coding Rna (Lncrna) In The Organization Of Nuclear Bodies, Soobin An

Symposium of Student Scholars

Nuclear bodies (NBs) (e.g., the nucleolus, nuclear speckles, and others) are membraneless compartments within the eukaryotic cell nucleus that selectively accumulate and retain specific nuclear proteins. NBs have become a new interest in recent discoveries because of their potential involvement in cancer and neurological disorders. However, the regulation and function of NBs are still enigmatic. Our laboratory studies a specific type of NBs, called B-bodies, to understand how NBs are formed and regulated in the nucleus. We hypothesized that long non-coding RNA (lncRNA) functions as a structural scaffold of NBs.

The B-body is a recently discovered NB expressed in the …

Spr-5; Met-2 Maternal Reprogramming Cooperates With The Dream Complex To Regulate Developmental Cell Fates, Jazmin Dozier, Sandra Nguyen, Brandon Carpenter

Symposium of Student Scholars

Histone methylation is a post-transcriptional modification to the N-terminal tails of histone core proteins that regulates DNA accessibility, and consequently, gene expression. Like DNA, histone methylation can be inherited between generations, and is highly regulated during embryonic development. At fertilization, histone methylation must undergo maternal reprogramming to reset the epigenetic landscape in the new zygote. During maternal reprogramming of histone methylation in the nematode, C. elegans, H3K4me (a modification associated with active transcription) is removed by the H3K4 demethylase, SPR-5, and H3K9me (a modification associated with transcriptional repression) is subsequently added by the histone methyltransferase, MET-2. Recently, it was …

Utilization Of Bioinformatics And Immunocytochemistry To Examine Gap Junction Expression In Breast Cancers Cells, Jasmine D. Carter, Giovanni Reyes, Abeeha K. Choudhary, Eric A. Albrecht

Symposium of Student Scholars

Utilization of Bioinformatics and Immunocytochemistry to Examine Gap Junction Expression in Breast Cancers Cells.

Jasmine D. Carter¹, Giovanni Reyes¹, Abeeha Choudhary² and Eric A. Albrecht¹

Breast cancer is known for its diverse clinical classifications and expressing different levels of membrane proteins such as ion channels and gap junctions. This diversity allows more variations in cell polarization, which can lead to enhanced directional ion fluxes in certain breast cancer subtypes. We utilized the interactive web portal UALCAN to evaluate the gene expression data of gap junctions, ion exchange channels and cytoskeletal proteins in breast cancer …

Tissue-Specific Diversity Of The Muscleblind Expression In Adult Flies., Davron Hanley, Anton Bryantsev

Symposium of Student Scholars

Department of Molecular and Cellular Biology, Kennesaw State University

The muscleblind (mbl) family of RNA-binding proteins regulates alternative splicing, determining mRNA transcript composition for various types of tissue, and has been implicated in myotonic dystrophy. The mbl gene is subject to alternative splicing in Drosophila, leading to multiple isoforms, and has several paralogs in humans. Mbl proteins vary significantly in length, although the significance of such diversity and the role of specific isoforms have not been fully explored.

Using immunofluorescence microscopy and polyclonal serum, we analyzed Mbl protein expression across adult Drosophila tissues. Mbl was detected in …

Complimenting A Chlamydomonas Reinhardtii Mutation Using Cell Penetrating Peptides, Matthew Mckenzie

Symposium of Student Scholars

Complimenting a Chlamydomonas reinhardtii Mutation Using Cell Penetrating Peptides

Matthew McKenzie and Jennifer L. Cooper

Chlamydomonas reinhardtii is a photosynthetic model organism most notable for its easily manipulatable genetics. C. reinhardtii uses flagella to swim and optimize its growth conditions in the light. We plan to use cell penetrating peptides (CPPs) to compliment C. reinhardtii that is affected with the IFT46 mutation. Cell penetrating peptides are short peptides that can move across a cell membrane. The novel CPP that we are using is called TaT-CaM. It consists of the trans-activator of transcription (TaT) and the calmodulin domain (CaM) that binds …

The Role Of Proneural Transcription Factor Ngn-1/Neurogenin During Caenorhabditis Elegans Embryonic Development, Michaela Diane Crego

Symposium of Student Scholars

Accurate control of neuronal cell identification and movement is crucial to embryonic development. Defects in this process can lead to neurodevelopmental disorders, creating an imperative for further study. The transcription factor neurogenin is required for multiple neurodevelopmental processes during vertebrate embryonic development and mutations in this gene underpin multiple human neurological disorders. Despite this, little is known about how this gene controls nervous system development and function. Neurogenin is deeply conserved across phyla. As such, we can investigate neurogenin function in simple systems such as the nematode Caenorhabditis elegans, which has a close ortholog of neurogenin, ngn-1. Previous work in …