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The Role Of Cytoplasmic Polyadenylation Element Binding Protein -2 (Cpeb-2) In Human Breast Cancer, Joshua Tordjman
The Role Of Cytoplasmic Polyadenylation Element Binding Protein -2 (Cpeb-2) In Human Breast Cancer, Joshua Tordjman
Electronic Thesis and Dissertation Repository
Cyclooxygenase-2 (COX-2) is overexpressed in 40-50% of breast cancers, and promotes tumour progression through increased proliferation, migration, invasion, Epithelial-to-Mesenchymal Transition (EMT), and induction of therapy-resistant Stem-Like-Cells (SLCs). COX-2 stimulates expression of two oncogenic and SLC-promoting microRNAs (miR-526b, miR-655), which simultaneously target one gene, Cytoplasmic Polyadenylation Element Binding Protein-2 (CPEB-2). Hypothesis: CPEB-2 is a tumour- and SLC-suppressing gene in breast cancer. Results: CPEB-2 knockout in a non-tumourigenic mammary epithelial cell line MCF10A demonstrated increases in proliferation, migration, invasion, EMT markers, SLC content, and VEGF-D expression. CPEB-2, an mRNA-binding translation-regulating protein, was found to regulate the translation of tumour suppressor p53. When …