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Full-Text Articles in Life Sciences
Micropropagation And Root Culture Of Turkish Endemic Astragalus Chrysochlorus (Leguminosae), Semra Hasançebi̇, Nesli̇han Turgut Kara, Özgür Çakir, Şule Ari
Micropropagation And Root Culture Of Turkish Endemic Astragalus Chrysochlorus (Leguminosae), Semra Hasançebi̇, Nesli̇han Turgut Kara, Özgür Çakir, Şule Ari
Turkish Journal of Botany
Efficient micropropagation and root culture protocols were developed for the endemic Astragalus chrysochlorus Boiss. & Kotschy. A high frequency of shoot formation (100%) and maximum multiplication (13 shoots per hypocotyl explant) were achieved on Murashige and Skoog (MS) media supplemented with 0.5 mg/L trans-Zeatin riboside (ZR). By using single regenerated hypocotyl explants, rooting was achieved at a rate of 93% on MS medium containing 2% sucrose without growth regulators. High frequency callus initiation and growth were achieved when single hypocotyl explants were inoculated on MS medium supplemented with 0.5 mg/L 2,4-Dichlorophenoxyacetic acid. Plant regeneration through indirect organogenesis was achieved on …
A New Onosma (Boraginaceae) Species From Southern Anatolia, Turkey, Zeki̇ Aytaç, Zafer Türkmen
A New Onosma (Boraginaceae) Species From Southern Anatolia, Turkey, Zeki̇ Aytaç, Zafer Türkmen
Turkish Journal of Botany
Onosma aksoyii Aytaç & Türkmen sp. nova (Sect. Haplotricha H.Riedl, Boraginaceae) is described and illustrated as a new species. It grows in a Pinus nigra forest on serpentine rocks in Southern Anatolia (Konya province). Diagnostic characters of related species are compared and discussed. The characteristic features of pollen, stomata structure, and hairs were studied using a scanning electron microscope. The distribution map of this new species is presented.
The Effect Of Thidiazuron On The In Vitro Shoot Development Of Endemic Astragalus Cariensis In Turkey, Semi̇ha Eri̇şen, Emi̇ne Atalay, Mustafa Yorgancilar
The Effect Of Thidiazuron On The In Vitro Shoot Development Of Endemic Astragalus Cariensis In Turkey, Semi̇ha Eri̇şen, Emi̇ne Atalay, Mustafa Yorgancilar
Turkish Journal of Botany
Thidiazuron (TDZ) is a cytokinin-like substance that has often been used for shoot regeneration in recent years. We observed the effect of TDZ on callus formation and shoot regeneration from leaf and petiole explants of the endemic species Astragalus cariensis Boiss. Explants were cultured on Murashige and Skoog (MS) media including TDZ (in concentrations of 0.2, 0.4, or 0.6 mg/L) or in combination with \alpha-naphthaleneacetic acid (NAA) (in concentrations of 0.2 or 1.0 mg/L). Significant variations in the frequency of callus formation and the morphogenesis of the callus were obtained depending on the explant type. Petiole explant was determined to …